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产品描述：人25羟基维生素D-1α羟化酶,线粒体(CYP27B1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYP27B1含量。CYP27B1，也称为25-羟基维生素D3 1-α-羟化酶，是细胞色素P450超家族的一员，参与维生素D的代谢过程。它催化25-羟基维生素D3转化为活性形式1,25-二羟基维生素D3，对钙磷代谢和免疫调节至关重要。研究显示，CYP27B1的表达与多种疾病如类风湿关节炎和佝偻病有关，其功能异常可能导致维生素D代谢障碍。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于维生素D代谢调控、骨代谢相关疾病模型、肾脏功能研究以及免疫调节机制等科研领域的分析需求，为探究其在慢性肾病、佝偻病及自身免疫性疾病中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：1alpha(OH)ase ELISA Kit; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase ELISA Kit; 25-hydroxyvitamin D-1 alpha hydroxylase ELISA Kit; 25-OHD-1 alpha-hydroxylase ELISA Kit; Calcidiol 1-monooxygenase ELISA Kit; CP27B_HUMAN ELISA Kit; CP2B ELISA Kit; CYP1 ELISA Kit; CYP1ALPHA ELISA Kit; CYP27B ELISA Kit; Cyp27b1 ELISA Kit; Cytochrome p450 27B1 ELISA Kit; Cytochrome p450 27B13 ELISA Kit; Cytochrome P450 family 27 subfamily B polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1 ELISA Kit; Cytochrome P450 subfamily XXVIIB polypeptide 1 ELISA Kit; Cytochrome P450C1 alpha ELISA Kit; Cytochrome P450VD1-alpha ELISA Kit; mitochondrial ELISA Kit; P450C1 alpha ELISA Kit; P450c1 ELISA Kit; P450C1-alpha ELISA Kit; P450VD1-alpha ELISA Kit; PDDR ELISA Kit; VD3 1A hydroxylase ELISA Kit; VDD1 ELISA Kit; VDDR ELISA Kit; VDDR I ELISA Kit; VDDRI ELISA Kit; VDR ELISA Kit
缩写：CYP27B1
Uniprot No.：O15528
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP27B1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-97 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of human CYP27B1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4722.3722.4222.24810001.7151.6151.6651.4915001.0881.0481.0680.8942500.7110.7010.7060.5321250.4040.3940.3990.22562.50.3100.3000.3050.13131.250.2490.2390.2440.07000.1750.1730.174 | ||||||
货期：3-5 working days
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