首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠细胞色素P4502E1(CYP2E1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYP2E1含量。CYP2E1是一种参与多种物质代谢的单加氧酶，在帕金森病和肝细胞癌（HCC）中发挥重要作用。其表达与活性影响疾病进程，通过代谢酒精产生氧自由基和毒性代谢产物，与多条信号通路相关，调控肝脏损伤和疾病发展。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研场景中肝脏代谢功能评估、药物诱导酶活性研究、毒理学模型构建以及酒精性肝病、非酒精性脂肪肝等疾病机制探索，为体外研究CYP2E1在代谢调控、氧化应激反应中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cyp2e1 ELISA kit; Cyp2e ELISA kit; Cyp2e-1Cytochrome P450 2E1 ELISA kit; EC 1.14.14.1 ELISA kit; 4-nitrophenol 2-hydroxylase ELISA kit; EC 1.14.13.n7 ELISA kit; CYPIIE1 ELISA kit; Cytochrome P450-J ELISA kit; Cytochrome P450RLM6 ELISA kit
缩写：CYP2E1
Uniprot No.：P05182
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 94-105 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 88-101 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 91-107 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
回收率：
| The recovery of rat CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 88-99 | |||||
| EDTA plasma (n=4) | 105 | 97-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.4312.3672.3992.21652.0151.9681.9921.8092.51.4311.4021.4171.2341.250.8760.8450.8610.6780.6250.5120.5340.5230.3400.3120.3640.3510.3580.1750.1560.2880.2830.2860.10300.1860.1800.183 | |||||||
货期：3-5 working days
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