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产品描述：人细胞色素P450 2E1(CYP2E1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYP2E1含量。CYP2E1是一种细胞色素P450酶。其在人体药物代谢和内源性物质合成中发挥重要作用。研究机制方面，它可催化多种底物代谢，参与生物转化过程。异常表达与氧化应激、药物毒性、疾病发生如肝癌等密切相关，是药物研发和疾病防治的重要靶点。试剂盒检测范围为43.75 pg/mL-2800 pg/mL，该产品适用于体外实验中的药理学研究、毒理学评估以及代谢通路调控机制探索，例如通过检测药物处理后的细胞或动物模型样本中CYP2E1表达变化，分析化合物对代谢酶活性的影响，或结合酒精暴露实验探究氧化应激相关分子机制。为研究肝脏代谢功能、外源性物质毒性作用机制以及相关疾病模型构建提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：4-nitrophenol 2-hydroxylase ELISA Kit; CP2E1_HUMAN ELISA Kit; CPE1 ELISA Kit; CYP2E ELISA Kit; Cyp2e1 ELISA Kit; CYPIIE1 ELISA Kit; Cytochrome P450 2E1 ELISA Kit; Cytochrome P450 isozyme 3A ELISA Kit; Cytochrome P450 LM3A ELISA Kit; Cytochrome P450; family 2; subfamily e; polypeptide 1 ELISA Kit; cytochrome P450; subfamily IIE (ethanol-inducible); polypeptide 1 ELISA Kit; Cytochrome P450; subfamily IIE ELISA Kit; Cytochrome P450-ALC ELISA Kit; Cytochrome P450-J ELISA Kit; Cytochrome P450RLM6 ELISA Kit; EC 1.14.13.- ELISA Kit; EC 1.14.13.n7 ELISA Kit; Ethanol-inducible P450 ELISA Kit; Flavoprotein-linked monooxygenase ELISA Kit; MGC133529 ELISA Kit; Microsomal monooxygenase ELISA Kit; OTTHUMP00000020813 ELISA Kit; OTTHUMP00000046571 ELISA Kit; P450 ALC ELISA Kit; P450-J ELISA Kit; P450C2E ELISA Kit; P450J ELISA Kit; P450RLM6 ELISA Kit; Xenobiotic monooxygenase ELISA Kit
缩写：CYP2E1
Uniprot No.：P05181
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：43.75 pg/mL-2800 pg/mL
灵敏度：10.94 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 85-98 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-94 | ||||
| EDTA plasma (n=4) | 97 | 95-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected28001.8301.9521.8911.79114001.0081.0761.0420.9427000.6360.6040.6200.5203500.3510.3690.3600.2601750.2180.2240.2210.12187.50.1610.1590.1600.06043.750.1230.1290.1260.02600.1030.0970.100 | ||||||
货期：3-5 working days
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