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产品描述：人细胞色素P450 2C9(CYP2C9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYP2C9含量。CYP2C9 是细胞色素 P450 酶家族重要成员。它参与众多药物的代谢过程，在药物体内消除和疗效发挥中起关键作用。研究机制主要围绕其基因多态性对药物代谢的影响，明确其与药物不良反应、个体差异等的关联，为精准用药提供依据。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品适用于药理学研究、药物代谢动力学分析、体外药物相互作用评估等领域，可为药物开发过程中酶活性调控研究、药物 - 酶结合特性分析及代谢毒性评价提供可靠工具，特别适合实验室开展肝脏代谢功能相关的基础研究或化合物筛选实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：(R)-limonene 6-monooxygenase ELISA Kit; (S)-limonene 6-monooxygenase ELISA Kit; (S)-limonene 7-monooxygenase ELISA Kit; CP2C9_HUMAN ELISA Kit; CPC9 ELISA Kit; CYP2C ELISA Kit; CYP2C10 ELISA Kit; CYP2C9 ELISA Kit; CYPIIC9 ELISA Kit; cytochrome P-450 S-mephenytoin 4-hydroxylase ELISA Kit; Cytochrome P-450MP ELISA Kit; Cytochrome P450 2C9 ELISA Kit; Cytochrome P450 MP-4 ELISA Kit; Cytochrome P450 MP-8 ELISA Kit; Cytochrome P450 PB-1 ELISA Kit; Cytochrome P450; family 2; subfamily C; polypeptide 9 ELISA Kit; Cytochrome p4502C9 ELISA Kit; flavoprotein-linked monooxygenase ELISA Kit; MGC149605 ELISA Kit; MGC88320 ELISA Kit; microsomal monooxygenase ELISA Kit; OTTHUMP00000020135 ELISA Kit; P450 MP ELISA Kit; P450 PB 1 ELISA Kit; P450 PB1 ELISA Kit; P450IIC9 ELISA Kit; P450MP ELISA Kit; S mephenytoin 4 hydroxylase ELISA Kit; S-mephenytoin 4-hydroxylase ELISA Kit; xenobiotic monooxygenase ELISA Kit
缩写：CYP2C9
Uniprot No.：P11712
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP2C9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 94-109 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 89-101 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 83-98 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 94-111 | ||||||
回收率：
| The recovery of human CYP2C9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 81-94 | |||||
| EDTA plasma (n=4) | 105 | 97-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 15002.4972.3892.4432.277 7501.7951.7671.7811.615 3751.0761.0621.0690.903 187.50.6050.5950.6000.434 940.3760.3660.3710.205 470.2480.2580.2530.087 23.50.2150.2110.2130.047 00.1690.1630.166 | |||||||
货期：3-5 working days
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