首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠细胞色素P450 2E1(CYP2E1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYP2E1含量。CYP2E1是细胞色素P450超家族成员，参与多种内源性和外源性物质代谢，与HCC、帕金森病等疾病相关。其表达受多种因素调控，研究机制涉及基因多态性、酶构型特征及代谢行为。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于体外研究中小鼠模型肝脏代谢功能评估、药物诱导的CYP2E1表达变化分析、毒理学研究中酶活性调控机制探索，以及酒精性肝病或氧化应激相关疾病的分子机制研究，为代谢通路及药物相互作用提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cyp2e1 ELISA kit; Cyp2e ELISA kit; Cyp2e-1Cytochrome P450 2E1 ELISA kit; EC 1.14.14.1 ELISA kit; 4-nitrophenol 2-hydroxylase ELISA kit; EC 1.14.13.n7 ELISA kit; CYPIIE1 ELISA kit; Cytochrome P450-ALC ELISA kit; Cytochrome P450-J ELISA kit
缩写：CYP2E1
Uniprot No.：Q05421
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-92 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-113 | ||||||
回收率：
| The recovery of mouse CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-100 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected25002.4822.6582.5702.38412501.9682.0762.0221.8366251.4181.5031.4611.2753120.8330.8570.8450.6591560.5550.5210.5380.352780.3150.3210.3180.132390.2350.2440.2400.05400.1830.1880.186 | |||||||
货期：3-5 working days
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