| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | DED; BFR2; CHE1; CHE-1 |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human AATF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇涉及AATF抗体的文献摘要概括(基于近年研究趋势模拟，具体文献需核实)：
1. **文献名称**：*AATF mediates anti-apoptotic signaling in triple-negative breast cancer through interaction with SMAR1*
**作者**：Bruno T, et al.
**摘要**：研究利用AATF特异性抗体进行免疫沉淀和Western blot分析，揭示AATF通过与SMAR1蛋白相互作用抑制乳腺癌细胞凋亡，并促进化疗耐药性的分子机制。
2. **文献名称**：*AATF/Che-1 regulates TOP2A expression and mediates sensitivity to etoposide in glioblastoma*
**作者**：Hassan M, et al.
**摘要**：通过AATF抗体进行染色质免疫沉淀(ChIP)和免疫组化实验，证明AATF通过直接结合TOP2A启动子调控其表达，影响胶质母细胞瘤对依托泊苷的敏感性。
3. **文献名称**：*AATF promotes prostate cancer cell survival by modulating the p53 signaling pathway*
**作者**：Zhang Y, et al.
**摘要**：利用AATF抗体进行免疫荧光和流式细胞术，发现AATF通过抑制p53转录活性减少前列腺癌细胞凋亡，可能成为潜在治疗靶点。
**注**：以上为模拟示例，实际文献需通过PubMed/Google Scholar以“AATF antibody” + “Western blot/immunohistochemistry”等关键词检索确认。
The AATF (Apoptosis Antagonizing Transcription Factor) antibody is a key tool for studying the multifunctional protein AATF, which plays critical roles in cellular homeostasis, stress response, and cancer biology. AATF, also known as CHE-1 or DED, is a 523-amino acid protein containing conserved domains like the leucine zipper and nuclear localization signals. It regulates apoptosis by interacting with p53. MDM2. and other pro-survival pathways, while also modulating transcription, cell cycle progression, and DNA damage repair.
AATF antibodies (typically rabbit or mouse monoclonal/polyclonal) enable researchers to detect and quantify AATF expression in various experimental models. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation to investigate AATF's subcellular localization, protein interactions, and expression patterns in normal vs. diseased tissues.
Given AATF's overexpression in cancers (e.g., prostate, breast, glioblastoma) and its links to chemoresistance, AATF antibodies have become essential for exploring its oncogenic mechanisms and therapeutic potential. Commercial AATF antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Proper controls, including isotype-matched antibodies and peptide blocking experiments, are recommended to ensure result reliability.
×
