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产品描述：猪半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CST3含量。CST3即半胱氨酸蛋白酶抑制剂C，在人体中广泛表达。它可抑制半胱氨酸蛋白酶的活性。在科研领域，其与神经系统疾病、肾脏疾病等方面研究较多，比如在神经退行性疾病中，CST3水平变化可能参与疾病发生发展机制。试剂盒检测范围为0.78 μg/mL-50 μg/mL，支持科研人员开展猪模型相关研究，如肾脏功能评估、代谢性疾病机制探索或药物干预效果验证等方向，尤其适用于动物实验样本中Cys-C表达水平的动态监测，为畜牧兽医学、比较医学及基础病理学研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CST3
Uniprot No.：Q0Z8R0
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 μg/mL-50 μg/mL
灵敏度：0.2 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 84-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of pig Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 9-103 | ||||
| EDTA plasma (n=4) | 93 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 500.2020.2050.204 250.3050.3070.306 12.50.4460.4310.439 6.250.6460.6580.652 3.120.8510.8630.857 1.561.5821.4971.540 0.782.0451.9732.009 02.7582.6042.681 | ||||||
货期：3-5 working days
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