首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠D二聚体(D2D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的D2D含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，广泛应用于心血管疾病模型、凝血功能障碍机制研究、药物作用评估等基础科研领域，尤其适合在小鼠模型中动态监测凝血系统活化程度或评价抗血栓药物干预效果，为实验室提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：D2D
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 87-103 | |||||
回收率：
| The recovery of mouse D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 92-105 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5192.4192.4692.30010001.8011.7011.7511.5825001.1221.0821.1020.9332500.6740.6640.6690.5001250.4380.4280.4330.26462.50.3550.3450.3500.18131.250.2890.2840.2870.11800.1700.1680.169 | ||||||
货期：3-5 working days
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