| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BSP; BNSP; SP-II; BSP-II |
| WB Predicted band size | 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human IBSP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于IBSP抗体的3篇参考文献，包含文献名称、作者及摘要概要：
---
1. **文献名称**: *"Bone sialoprotein and its osteoclast adhesion molecule in bone metastasis"*
**作者**: Fisher LW, et al.
**摘要**: 研究探讨了IBSP(骨唾液酸蛋白)作为整合素结合蛋白在骨微环境中的作用，发现其抗体可特异性阻断破骨细胞与骨基质的黏附，提示其在抑制肿瘤骨转移中的潜在治疗价值。
---
2. **文献名称**: *"Development of monoclonal antibodies against human bone sialoprotein: immunohistochemical characterization in bone metastases"*
**作者**: Karadag A, et al.
**摘要**: 报道了针对人IBSP的单克隆抗体的开发与验证，通过免疫组化证实该抗体在前列腺癌骨转移组织中高表达，支持IBSP作为骨转移诊断的生物标志物。
---
3. **文献名称**: *"Role of bone sialoprotein in the recruitment of malignant stem cells to the osteogenic niche"*
**作者**: Bellahcène A, et al.
**摘要**: 利用IBSP抗体阻断实验，揭示了肿瘤干细胞通过IBSP-整合素通路定植于骨龛的机制，为靶向IBSP的抗体疗法抑制癌症骨转移提供了理论依据。
---
如需更多文献或具体应用场景，可进一步补充关键词细化检索范围。
IBSP (Integrin-Binding Sialoprotein), also known as bone sialoprotein II, is a secreted glycoprotein predominantly expressed in mineralized tissues, including bone, dentin, and cementum. It belongs to the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family and plays a critical role in bone metabolism, particularly in osteoblast-mediated matrix mineralization and osteoclast regulation. Structurally, IBSP contains an Arg-Gly-Asp (RGD) motif that enables binding to integrin receptors (e.g., αvβ3) on cell surfaces, facilitating cell-matrix interactions. It also interacts with hydroxyapatite crystals, promoting biomineralization.
IBSP antibodies are essential tools in studying skeletal development, bone remodeling, and pathologies like osteoporosis or cancer bone metastasis. In oncology, IBSP is overexpressed in bone-homing cancers (e.g., breast, prostate), making its antibodies valuable for detecting metastatic potential. Researchers use IBSP antibodies in techniques like Western blot, immunohistochemistry (IHC), and immunofluorescence (IF) to localize and quantify IBSP expression in tissues or cell cultures.
Commercial IBSP antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Specificity validation via knockout controls or siRNA knockdown is crucial due to potential cross-reactivity with other SIBLING proteins. Recent studies also explore IBSP's role in tissue engineering and regenerative medicine, emphasizing its dual function in mineralization and cell signaling.
×
