| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PTPRC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于PTPRC抗体的3篇参考文献及其摘要概括：
1. **《CD45: a critical regulator of signaling thresholds in immune cells》**
*作者：Hermiston ML, et al.*
**摘要**：该综述系统总结了CD45(PTPRC)在免疫细胞信号调控中的核心作用，重点讨论了其通过酪氨酸磷酸酶活性调节T细胞和B细胞受体信号通路的机制，并提及多种抗CD45抗体在实验中的应用。
2. **《Monoclonal antibody to the common leukocyte antigen (CD45) inhibits human neutrophil chemotaxis》**
*作者：Zhou J, et al.*
**摘要**：研究通过特异性抗CD45抗体(克隆号HI30)阻断中性粒细胞表面CD45.发现其显著抑制细胞趋化运动，表明CD45在炎症反应中通过调节细胞骨架重组参与迁移调控。
3. **《CD45 isoform expression in human lymphomas》**
*作者：Bourguin A, et al.*
**摘要**：利用多克隆抗CD45抗体对淋巴瘤组织进行免疫组化分析，揭示了不同CD45剪接异构体在淋巴瘤亚型中的差异表达，提示其在肿瘤微环境诊断中的潜在价值。
The **PTPRC antibody** targets the Protein Tyrosine Phosphatase Receptor Type C (PTPRC), commonly known as CD45. a transmembrane glycoprotein expressed on all nucleated hematopoietic cells. CD45 is a key regulator of immune cell signaling, functioning as a tyrosine phosphatase that modulates the activity of Src-family kinases (e.g., Lck, Fyn) by dephosphorylating inhibitory C-terminal residues. This activity is critical for T-cell and B-cell receptor signaling, influencing immune cell development, activation, and differentiation.
CD45 exists as multiple isoforms (e.g., CD45RA, CD45RO) generated by alternative splicing, which differ in extracellular domains and are associated with distinct immune cell subsets. For example, CD45RA is typically expressed on naïve T-cells, while CD45RO marks antigen-experienced memory T-cells. Antibodies against PTPRC are widely used in research and diagnostics to identify and characterize leukocyte populations via flow cytometry, immunohistochemistry, or Western blotting. They help distinguish immune cell lineages (e.g., T-cells vs. B-cells) and activation states, aiding studies in immunology, oncology, and autoimmune diseases.
Clinically, altered CD45 expression or splicing is linked to hematologic malignancies (e.g., leukemia) and immunodeficiency disorders. PTPRC antibodies are employed in diagnostic panels to classify leukemias/lymphomas and monitor disease progression. Their specificity makes them indispensable tools for understanding immune dysregulation and developing targeted therapies.
×
