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Human Defensin-6(DEFA6) ELISA kit

  • 中文名： 人防御素6(DEFA6)酶联免疫试剂盒
货号: IPD-EL006658HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：人防御素6(DEFA6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DEFA6含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于探究肠道疾病模型、病原体感染或免疫调控实验中DEFA6的表达动态，为研究其抗菌机制、黏膜免疫应答及宿主 - 微生物互作提供可靠工具，特别适用于体外实验的蛋白质水平检测及生物标志物筛选等科研用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：alpha 6 ELISA Kit; DEF6 ELISA Kit; DEF6_HUMAN ELISA Kit; DEFA 6 ELISA Kit; DEFA6 ELISA Kit; Defensin 6 ELISA Kit; Defensin alpha 6 ELISA Kit; Defensin alpha 6 Paneth cell specific ELISA Kit; Defensin ELISA Kit; Defensin-6 ELISA Kit; HD 6 ELISA Kit; HD6 ELISA Kit

  • 缩写：DEFA6

  • Uniprot No.：Q01524

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：0.156 ng/mL-10 ng/mL

  • 灵敏度：0.039 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Immunology

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %95

    Range %91-99

    1:2Average %103

    Range %98-106

    1:4Average %110

    Range %106-114

    1:8Average %92

    Range %89-97

  • 回收率：

    The recovery of human DEFA6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)8481-89

    EDTA plasma (n=4)9793-100

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/mlOD1OD2AverageCorrected  102.2172.1042.1612.047  51.7621.7521.7571.643  2.51.2531.1981.2261.112  1.250.8490.8560.8530.739  0.6250.5280.5340.5310.417  0.3120.3540.3680.3610.247  0.1560.2440.2380.2410.127  00.1150.1120.114　  







  • 货期：3-5 working days

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