首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠中性粒细胞防御素4(DEFA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DEFA4含量。DEFA4是一种靶点。它在生物体内可能具有特定生理功能。目前对于它的研究，主要聚焦于其在相关生理和病理过程中的作用机制，探索它与疾病发生发展的联系，为开发针对该靶点的治疗手段、药物等提供理论依据。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域中小鼠模型感染性疾病机制研究、炎症反应评估或免疫调控实验，例如通过检测不同病理状态下DEFA4的表达水平变化，探索其在宿主防御系统中的作用，亦可用于药物开发中免疫调节剂的效果验证。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Defa4 ELISA kit; Defcr4Alpha-defensin 4 ELISA kit; Defensin-related cryptdin-4 ELISA kit
缩写：DEFA4
Uniprot No.：P28311
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse DEFA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:2 | Average % | 93 | |||||
| Range % | 90-96 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 97-105 | ||||||
回收率：
| The recovery of mouse DEFA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 95-104 | |||||
| EDTA plasma (n=4) | 83 | 80-86 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.6542.6892.6722.515102.0592.0012.0301.87351.3121.3351.3241.1672.50.8520.8680.8600.7031.250.5050.5120.5090.3520.6250.3730.3600.3670.2100.3120.2660.2680.2670.11000.1580.1560.157 | |||||||
货期：3-5 working days
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