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产品描述：人DNA损伤诱导转录因子4样蛋白(DDIT4/RTP801)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、细胞裂解物、尿液、脑脊液（CSF）样本中的DDIT4含量。DDIT4/RTP801是一种应激蛋白，最初在果蝇中作为TOR信号通路和细胞大小负调控因子被发现。它在哺乳动物中与mTOR信号通路相互作用，作为其上游抑制剂。DDIT4/RTP801通过结合ATP合成酶的α亚基来抑制线粒体代谢，并诱导细胞凋亡，从而在肿瘤生长中发挥抑制作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础医学研究中探索该蛋白在疾病发生发展中的作用机制，例如评估癌症模型中的 DNA 损伤应答、神经系统疾病中的氧化应激水平变化，或药物干预实验中生物标志物的动态监测。满足科研工作者在分子机制研究、生物标志物筛选及药物开发等领域的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DDIT4 ELISA Kit; DDIT4_HUMAN ELISA Kit; Dig2 ELISA Kit; DNA damage inducible transcript 4 ELISA Kit; DNA damage inducible transcript 4 protein ELISA Kit; DNA damage-inducible transcript 4 protein ELISA Kit; FLJ20500 ELISA Kit; HIF 1 responsive protein RTP801 ELISA Kit; HIF 1 responsive RTP801 ELISA Kit; HIF-1 responsive protein RTP801 ELISA Kit; Protein regulated in development and DNA damage response 1 ELISA Kit; REDD-1 ELISA Kit; REDD1 ELISA Kit; RTP801 ELISA Kit
缩写：DDIT4
Uniprot No.：Q9NX09
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates, cell lysates, urine, cerebrospinal fluid (CSF)
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DDIT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human DDIT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.9482.7542.8512.69152.5392.4122.4762.3162.51.7811.6621.7221.5621.251.2481.1981.2231.0630.6250.7380.7060.7220.5620.3120.5140.5040.5090.3490.1560.3050.2980.3020.14200.1620.1580.160 | ||||||
货期：3-5 working days
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