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Rabbit Polyclonal CDC27 Antibody

  • 中文名: CDC27抗体
  • 别    名: APC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E
货号: IPDX11649
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/5000 Human,Mouse,Rat

产品详情

AliasesAPC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E
WB Predicted band size92 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human CDC27
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于CDC27抗体的3篇参考文献及其摘要概述:

1. **文献名称**: "Identification of cdc27 as a component of the anaphase-promoting complex"

**作者**: King, R.W., Peters, J.M., Tugendreich, S., et al.

**摘要**: 该研究通过免疫沉淀和Western blot技术,首次将CDC27鉴定为后期促进复合体(APC/C)的核心组分,并开发了特异性抗体用于验证其在酵母和人类细胞中的保守功能,揭示了CDC27在细胞周期调控中的关键作用。

2. **文献名称**: "Phosphorylation of the APC subunit Cdc27 by mitotic kinases"

**作者**: Peters, J.M., King, R.W., Höög, C., et al.

**摘要**: 利用CDC27特异性抗体,研究揭示了CDC27在有丝分裂期间被周期蛋白依赖性激酶(CDK)磷酸化的机制,通过免疫荧光和免疫印迹分析,证实其磷酸化状态对APC/C活性和细胞周期进程的调控至关重要。

3. **文献名称**: "CDC27 expression in colorectal cancer: correlation with clinicopathological parameters and prognosis"

**作者**: Yu, H., Liu, X., Huang, J., et al.

**摘要**: 该研究通过免疫组化技术分析结直肠癌组织中CDC27蛋白的表达水平,发现其高表达与肿瘤分期和不良预后显著相关,提示CDC27可能作为潜在的癌症生物标志物和治疗靶点。

**备注**:以上文献均为示例,实际引用需根据具体研究内容核实。建议通过PubMed或Google Scholar以“CDC27 antibody”或“APC/C CDC27 function”等关键词检索最新文献。

背景信息

The CDC27 antibody targets the CDC27 protein, a critical component of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for cell cycle regulation. CDC27 (also known as APC3) functions as a scaffold protein within the APC/C, facilitating its assembly and interaction with co-activators like CDC20 and CDH1. The APC/C governs progression through mitosis and the G1 phase by targeting key cell cycle regulators, such as securin and cyclin B, for ubiquitin-mediated proteasomal degradation, ensuring timely transitions between cell cycle phases. Antibodies against CDC27 are widely used in research to study APC/C structure, function, and dynamics during cell division. They are applied in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess APC/C activity, subunit composition, or localization in various cellular contexts. Dysregulation of CDC27 or APC/C is linked to genomic instability, mitotic errors, and cancer, making these antibodies valuable tools in oncology and cell biology studies. Additionally, CDC27 antibodies help investigate checkpoint mechanisms, DNA damage responses, and potential therapeutic targets for diseases involving cell cycle aberrations. Commercial CDC27 antibodies are typically validated for specificity across human, mouse, and rat models.

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