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产品描述：人中性粒细胞防御素3(DEFA3)酶联免疫试剂盒检测血清、血浆,组织匀浆样本中的DEFA3含量。DEFA3是一种防御素，存在于人体中性粒细胞中，具有抗菌、抗病毒和免疫调节等功能。研究机制方面，其通过与病原体相互作用，破坏病原体的膜结构，影响其生存与繁殖，还能调节免疫细胞活性，在机体抗感染免疫中发挥重要作用。试剂盒检测范围为1.87 ng/mL-120 ng/mL，本产品适用于科研领域对DEFA3表达水平的定量研究，例如探索感染性疾病中宿主防御机制、炎症相关疾病的分子调控路径，或评估不同实验条件下中性粒细胞功能状态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DEFA3 ELISA Kit; DEF3Neutrophil defensin 3 ELISA Kit; Defensin ELISA Kit; alpha 3 ELISA Kit; HNP-3 ELISA Kit; HP-3 ELISA Kit; HP3) [Cleaved into: HP 3-56; Neutrophil defensin 2 ELISA Kit; HNP-2 ELISA Kit; HP-2 ELISA Kit; HP2)] ELISA Kit
缩写：DEFA3
Uniprot No.：P59666
种属：Homo sapiens (Human)
样本类型：serum, plasma,tissue homogenates
检测范围：1.87 ng/mL-120 ng/mL
灵敏度：0.47 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 97 | |
| Range % | 88-101 | ||
| 1:2 | Average % | 92 | |
| Range % | 84-96 | ||
| 1:4 | Average % | 94 | |
| Range % | 85-99 | ||
| 1:8 | Average % | 98 | |
| Range % | 90-102 |
回收率：
| The recovery of human DEFA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 89-104 | ||||
| EDTA plasma (n=4) | 98 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1201.8751.8971.8861.762 601.4121.4761.4441.320 300.9070.9280.9180.794 150.5520.5450.5490.425 7.50.3590.3440.3520.228 3.750.2450.2410.2430.119 1.870.1850.1790.1820.058 00.1250.1230.124 | ||||||
货期：3-5 working days
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