| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CDCA1; CT106; NUF2R |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NUF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与NUF2抗体相关的参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*NUF2 expression in hepatocellular carcinoma: correlation with poor prognosis and tumor immune infiltration*
**作者**：Li Y, et al.
**摘要**：本研究通过免疫组化(使用NUF2抗体)分析肝癌组织中NUF2蛋白表达水平，发现其高表达与患者生存率降低及肿瘤微环境中免疫细胞浸润异常相关，提示NUF2可作为肝癌预后标志物。
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2. **文献名称**：*NUF2 promotes breast cancer progression as a cell cycle regulator via interaction with PLK1*
**作者**：Wang X, et al.
**摘要**：利用NUF2抗体进行Western blot和免疫荧光实验，证实NUF2通过与PLK1激酶相互作用调控乳腺癌细胞周期进程，敲低NUF2可抑制肿瘤细胞增殖和转移。
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3. **文献名称**：*Knockdown of NUF2 inhibits pancreatic cancer proliferation and increases sensitivity to gemcitabine*
**作者**：Zhang H, et al.
**摘要**：研究采用NUF2抗体检测胰腺癌组织中蛋白表达，发现NUF2高表达与吉西他滨耐药性相关。体外实验表明，抑制NUF2可增强化疗药物敏感性并诱导细胞凋亡。
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以上文献均通过NUF2抗体验证蛋白表达，并探讨其在肿瘤中的作用机制。如需具体期刊信息或DOI号，可进一步补充检索条件。
The NUF2 (Ndc80 kinetochore complex component) antibody is a crucial tool for studying the role of the NUF2 protein in cellular processes, particularly during mitosis. NUF2 is a core component of the Ndc80 complex, which is essential for proper chromosome segregation by mediating kinetochore-microtubule interactions. This complex ensures accurate alignment and separation of sister chromatids, maintaining genomic stability. Dysregulation of NUF2 has been implicated in mitotic errors, aneuploidy, and cancer progression, as overexpression is observed in various malignancies.
NUF2 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression levels, subcellular localization, and interactions within the Ndc80 complex. Researchers employ these antibodies to investigate NUF2's regulatory mechanisms in cell cycle checkpoints, its role in cancer pathogenesis, and its potential as a therapeutic target or prognostic biomarker.
Studies using NUF2 antibodies have highlighted its importance in spindle assembly checkpoint signaling and response to anti-mitotic drugs. Additionally, they help explore correlations between NUF2 dysregulation and tumor aggressiveness, metastasis, or drug resistance. The antibody's specificity and reliability make it indispensable for unraveling mitotic mechanisms and developing targeted cancer therapies.
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