首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β-防御素1酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DEFB1含量。DEFB1（防御素β1）是一种抗菌肽，属于β-防御素家族，由上皮细胞高度表达。其编码的蛋白质具有抗菌活性，对上皮表面抵抗微生物定植起重要作用。研究显示，DEFB1在先天免疫反应中发挥关键作用，与哮喘、严重脓毒症等疾病的发生机制有关。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本产品适用于科研领域探索hBD - 1在病原体感染模型、炎症反应机制或组织微环境调控中的动态变化，为免疫调控研究及药物开发提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BD 1 ELISA Kit; BD-1 ELISA Kit; BD1 ELISA Kit; beta 1 ELISA Kit; Beta defensin 1 ELISA Kit; Beta-defensin 1 ELISA Kit; DEFB 1 ELISA Kit; DEFB1 ELISA Kit; DEFB1_HUMAN ELISA Kit; DEFB101 ELISA Kit; Defensin ELISA Kit; Defensin beta 1 ELISA Kit; Defensin beta 1 preproprotein ELISA Kit; HBD 1 ELISA Kit; hBD-1 ELISA Kit; HBD1 ELISA Kit; MGC51822 ELISA Kit
缩写：DEFB1
Uniprot No.：P60022
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Microbiology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:10 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:40 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-99 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.5442.5202.5322.4121001.8791.7601.8201.700501.1651.0841.1251.005250.6780.6390.6590.53912.50.4430.4240.4340.3146.250.3250.3090.3170.1973.120.1980.1890.1940.07400.1230.1170.120 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BD-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled BD-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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