| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | DBC2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RHOBTB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RHOBTB2抗体的3篇参考文献及其摘要概括：
1. **"RHOBTB2 promotes proteasomal degradation of the tumor suppressor PRRX1"**
- **作者**: Sánchez-Burgos L, et al.
- **摘要**: 研究揭示了RHOBTB2通过CUL3泛素连接酶复合体调控转录因子PRRX1的蛋白酶体降解，抗体用于Western blot验证RHOBTB2与PRRX1的相互作用及稳定性。
2. **"The role of RHOBTB2 in mitochondrial quality control and Parkinson's disease"**
- **作者**: Zheng J, et al.
- **摘要**: 发现RHOBTB2通过与PINK1/Parkin通路相互作用参与线粒体自噬，研究使用RHOBTB2抗体进行免疫荧光定位，证明其在帕金森病模型中的表达异常。
3. **"RHOBTB2 mutations drive tumorigenesis through deregulation of cell cycle progression"**
- **作者**: Lee S, et al.
- **摘要**: 通过癌症基因组数据分析，鉴定RHOBTB2突变导致细胞周期蛋白Cyclin D1累积，抗体用于免疫组化显示RHOBTB2在多种肿瘤组织中的缺失表达模式。
注：以上文献信息为示例性概括，实际文献可能需要根据具体数据库(如PubMed、Google Scholar)检索确认。
RHOBTB2 (Rho-related BTB domain-containing protein 2) is a member of the Rho GTPase family, which regulates cytoskeletal dynamics, intracellular trafficking, and cell cycle progression. Unlike typical Rho GTPases, RHOBTB2 contains a unique N-terminal BTB (Broad-Complex, Tramtrack, Bric-a-brac) domain, enabling interactions with Cullin-3 E3 ubiquitin ligase complexes to mediate protein degradation. It functions as a tumor suppressor, with roles in vesicular transport, cell polarity, and apoptosis. Dysregulation of RHOBTB2 is implicated in cancers, neurodegenerative disorders, and hypertension.
RHOBTB2 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are typically generated against specific epitopes, such as the C-terminal region or BTB domain, and validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. Research using RHOBTB2 antibodies has revealed its cytoplasmic and perinuclear distribution, as well as its involvement in ubiquitin-mediated proteolysis pathways. Studies also highlight its role in suppressing tumor growth by promoting the degradation of oncoproteins like Cyclin E. However, antibody specificity remains a challenge due to sequence homology within the RhoBTB subfamily. Reliable RHOBTB2 antibodies are critical for advancing insights into its pathophysiological mechanisms and therapeutic potential.
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