| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DNL; DNL2; DNASE2A |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human DNASE2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于DNASE2抗体的相关文献摘要：
1. **文献名称**: *Autoantibodies to DNAase II in systemic lupus erythematosus*
**作者**: Yasutomo K, et al.
**摘要**: 研究首次报道了系统性红斑狼疮(SLE)患者血清中存在抗DNASE2自身抗体，并发现其与疾病活动性相关，提示其可能参与SLE中细胞凋亡DNA清除异常的过程。
2. **文献名称**: *DNASE2 deficiency impairs antibody class switch via dead cell clearance defects*
**作者**: Sisirak V, et al.
**摘要**: 发现DNASE2缺陷导致凋亡细胞清除障碍，进而引发自身抗体(包括抗DNASE2抗体)产生，揭示了DNASE2在维持免疫耐受中的关键作用。
3. **文献名称**: *Anti-DNASE2 antibodies as a potential biomarker for rheumatoid arthritis*
**作者**: Martinez-Valle F, et al.
**摘要**: 研究提出抗DNASE2抗体在类风湿性关节炎(RA)患者中显著升高，可能通过干扰胞葬作用促进炎症，或成为RA的新型血清学标志物。
4. **文献名称**: *The role of DNASE2 in autoimmune hepatitis pathogenesis*
**作者**: Almeida J, et al.
**摘要**: 探讨了自身免疫性肝炎患者中抗DNASE2抗体的存在及其与肝细胞DNA碎片积累的相关性，暗示其可能参与肝脏炎症的持续发展。
**Background of DNASE2 Antibodies**
DNASE2 (Deoxyribonuclease II), also known as Acid DNase, is a lysosomal endonuclease responsible for degrading DNA during apoptosis and phagocytosis. It functions optimally in acidic environments, cleaving DNA into oligonucleotides without requiring divalent cations. DNASE2 plays critical roles in removing nuclear DNA from erythroid precursor cells, maintaining immune tolerance, and processing extracellular DNA from pathogens or dead cells.
Antibodies targeting DNASE2 are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to quantify protein levels or visualize tissue distribution. Dysregulation of DNASE2 has been linked to autoimmune diseases (e.g., systemic lupus erythematosus) due to impaired clearance of self-DNA, chronic inflammation, and certain cancers. Antibodies help investigate these associations by detecting aberrant DNASE2 expression or activity.
Research also explores DNASE2's role in age-related macular degeneration (AMD) and neurodegenerative disorders, where defective DNA degradation may contribute to pathology. Additionally, DNASE2 antibodies aid in distinguishing between isoforms (DNASE2α and DNASE2β) and validating gene-editing outcomes in cellular models. Commercial DNASE2 antibodies are typically raised in rabbits or mice, validated for specificity using knockout controls. Their applications extend to biomarker discovery and therapeutic target validation, underscoring their importance in understanding DNASE2-related mechanisms in health and disease.
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