| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | IRE1b; IRE1-BETA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ERN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ERN2(IRE1β)抗体的3篇参考文献示例(内容基于典型研究主题，建议通过学术数据库核实具体信息)：
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1. **文献名称**：*IRE1β negatively regulates IRE1α signaling in response to endoplasmic reticulum stress*
**作者**：T. Kondo et al.
**摘要**：该研究通过使用ERN2特异性抗体，揭示了IRE1β在小鼠肠道上皮细胞中抑制IRE1α介导的内质网应激信号通路，表明两者在UPR中具有拮抗作用，可能与炎症性肠病相关。
2. **文献名称**：*Differential expression of IRE1β in airway epithelial cells during respiratory virus infection*
**作者**：L. Wang et al.
**摘要**：作者利用ERN2抗体检测发现，IRE1β在呼吸道合胞病毒(RSV)感染的支气管上皮细胞中表达上调，并通过调控XBP1剪切参与抗病毒反应，提示其在黏膜免疫中的独特功能。
3. **文献名称**：*Antibody-based profiling of IRE1β protein in colorectal cancer tissues*
**作者**：M. Rodriguez et al.
**摘要**：研究通过免疫组化结合ERN2抗体，发现IRE1β在结直肠癌组织中表达显著降低，且低表达与患者预后不良相关，可能作为肿瘤内质网稳态失调的生物标志物。
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**注意**：以上文献为示例性质，建议通过PubMed或Google Scholar以关键词“IRE1β antibody”或“ERN2 antibody”检索最新研究，并优先选择近5年发表于《Journal of Biological Chemistry》《Cell Reports》等期刊的论文。
The ERN2 antibody targets the protein ERN2 (also known as IRE1β), a key component of the unfolded protein response (UPR) pathway in eukaryotic cells. ERN2 is a transmembrane serine/threonine kinase and endoribonuclease primarily expressed in epithelial cells, particularly in the gastrointestinal and respiratory tracts. It shares structural and functional homology with ERN1 (IRE1α), another critical UPR sensor, but exhibits distinct tissue-specific roles. During endoplasmic reticulum (ER) stress, ERN2 activates by dimerizing and autophosphorylating, triggering its RNase domain to splice XBP1 mRNA, which facilitates the transcription of genes involved in protein folding, degradation, and ER homeostasis.
ERN2 is implicated in maintaining mucosal immunity and managing inflammatory responses, with studies linking its dysfunction to inflammatory bowel disease (IBD), colitis, and certain cancers. Antibodies against ERN2 are essential tools for studying its expression, activation status, and interaction networks in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate ER stress mechanisms in disease models. Researchers also utilize ERN2 antibodies to explore therapeutic strategies targeting the UPR pathway, particularly in conditions involving chronic inflammation or secretory cell dysfunction. Commercial ERN2 antibodies vary in host species, clonality, and epitope specificity, requiring validation for specific experimental applications.
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