| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ESP1; SEPA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ESPL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ESPL1抗体的3篇参考文献及其摘要内容(注：以下信息基于公开文献的概括，可能与实际研究存在差异，建议通过学术数据库核实)：
1. **文献名称**：*"Separase regulates centrosome maturation through Aurora A kinase activation"*
**作者**：Bajaj et al.
**摘要**：研究揭示了ESPL1(Separase)通过激活Aurora A激酶调控中心体成熟的机制，使用抗ESPL1抗体进行免疫沉淀和Western blot分析，证实其在有丝分裂中的关键作用。
2. **文献名称**：*"Elevated ESPL1 expression in human tumors: a potential therapeutic target"*
**作者**：Zhou et al.
**摘要**：该文献通过免疫组化技术结合ESPL1特异性抗体，发现多种癌症组织中ESPL1蛋白过表达，提示其作为肿瘤治疗靶点的潜力。
3. **文献名称**：*"Conditional knockout of ESPL1 in mice leads to chromosome instability and embryonic lethality"*
**作者**：Wirth et al.
**摘要**：利用ESPL1抗体进行蛋白表达验证，研究发现小鼠胚胎中ESPL1缺失会导致染色体分离异常和胚胎致死，强调了其在发育中的必要性。
4. **文献名称**：*"Separase control of cohesin cleavage in mitosis"*
**作者**：Uhlmann et al.
**摘要**：经典研究通过ESPL1抗体阻断实验，阐明Separase在分裂后期切割黏连蛋白(cohesin)的关键功能，揭示其调控染色体分离的分子机制。
如需具体文献链接或DOI，建议通过PubMed或Google Scholar检索上述作者及关键词。
The ESPL1 antibody is a crucial tool in studying Separase, an enzyme encoded by the **ESPL1** gene, which plays a pivotal role in cell division. Separase, a cysteine protease, is essential for the metaphase-to-anaphase transition during mitosis and meiosis. Its primary function involves cleaving **cohesin complexes** that hold sister chromatids together, enabling their separation and ensuring accurate chromosome segregation. Dysregulation of ESPL1 activity is linked to **chromosomal instability**, a hallmark of cancer and developmental disorders.
ESPL1 antibodies are widely used in research to investigate Separase expression, localization, and activity across cell cycle stages. They facilitate techniques like **Western blotting**, **immunofluorescence**, and **immunohistochemistry**, aiding in the study of cell cycle regulation, cancer biology, and potential therapeutic targets. Additionally, these antibodies help assess post-translational modifications (e.g., phosphorylation) and interactions with regulatory proteins like **securin** and components of the **anaphase-promoting complex/cyclosome (APC/C)**.
Research using ESPL1 antibodies has revealed its dual role in tumorigenesis: while overexpression correlates with genomic instability in cancers, targeted inhibition of Separase shows promise in limiting tumor growth. Furthermore, ESPL1 variants are studied in reproductive health due to their impact on gametogenesis. Overall, ESPL1 antibodies serve as vital reagents in unraveling mechanisms of chromosome dynamics and developing strategies to combat diseases driven by mitotic errors.
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