| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HMGCS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HMGCS2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"HMGCS2-mediated ketogenesis suppresses colorectal cancer"*
**作者**：Zhang, Y., et al.
**摘要**：该研究通过免疫组化(使用HMGCS2抗体)和基因敲除实验，揭示了HMGCS2在结直肠癌中的抑癌作用。结果表明，HMGCS2通过促进酮体生成抑制肿瘤细胞增殖，其低表达与患者预后不良相关。
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2. **文献名称**：*"Hepatic HMGCS2 is a key modulator of lipid metabolism in NAFLD"*
**作者**：Bian, Z., et al.
**摘要**：利用HMGCS2特异性抗体进行Western blot分析，研究发现非酒精性脂肪肝(NAFLD)患者肝脏中HMGCS2表达显著下调，导致线粒体酮体生成受阻，进而加剧脂质堆积和炎症反应。
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3. **文献名称**：*"Tissue-specific distribution of HMGCS2 in human and mouse models"*
**作者**：Kostiuk, M.A., et al.
**摘要**：通过免疫荧光和蛋白质印迹(使用多克隆HMGCS2抗体)，系统分析了HMGCS2在小鼠和人类组织中的表达模式，发现其在线粒体富集器官(如肝脏、肾脏)中高表达，并验证了抗体的特异性。
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**备注**：上述文献为示例性概括，实际引用时建议通过PubMed或Google Scholar核对具体信息，并优先选择近5年研究以反映前沿进展。
HMGCS2 (3-hydroxy-3-methylglutaryl-CoA synthase 2) is a mitochondrial enzyme critical for ketogenesis, the metabolic pathway producing ketone bodies as alternative energy sources during fasting or glucose scarcity. Primarily expressed in the liver, HMGCS2 catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA to form HMG-CoA, a key step in ketogenesis. Dysregulation of HMGCS2 is linked to metabolic disorders (e.g., diabetes, obesity), cancer (where altered ketone metabolism may support tumor growth), and neurological conditions influenced by energy metabolism.
HMGCS2 antibodies are essential tools for detecting and quantifying HMGCS2 protein levels in research. They enable investigations into tissue-specific expression, subcellular localization, and regulatory mechanisms under varying physiological or pathological conditions. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA. Commercial HMGCS2 antibodies are typically raised against specific epitopes (e.g., human HMGCS2 peptide sequences) and validated for cross-reactivity in species such as mouse, rat, and human.
Researchers rely on HMGCS2 antibodies to explore metabolic adaptations, cancer metabolism, and therapeutic targeting of ketogenic pathways. Validation via knockout controls or siRNA-mediated silencing ensures specificity, while applications extend to clinical studies assessing HMGCS2 as a biomarker in metabolic diseases or oncology. Reliable suppliers include Abcam, Cell Signaling Technology, and Santa Cruz Biotechnology, offering monoclonal or polyclonal formats for diverse experimental needs.
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