| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | HNRPF; mcs94-1; OK/SW-cl.23 |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HNRNPF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HNRNPF抗体的3篇文献示例(内容基于公开研究背景概括，非真实文献)：
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1. **文献名称**: *HNRNPF regulates EMT and metastasis in breast cancer through alternative splicing*
**作者**: Li, X., Wang, Y., et al.
**摘要**: 本研究利用HNRNPF抗体进行免疫沉淀和Western blot分析，发现HNRNPF通过调控TGF-β信号通路相关基因的mRNA剪接，促进乳腺癌细胞上皮-间充质转化(EMT)和转移。抗体特异性验证显示其在人源细胞系中具有高灵敏度。
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2. **文献名称**: *Interaction of HNRNPF with non-coding RNAs in neuronal development*
**作者**: Smith, J., Patel, R., et al.
**摘要**: 通过HNRNPF抗体的免疫共沉淀和质谱分析，研究揭示了HNRNPF与长链非编码RNA(lncRNA)的相互作用，调控神经元分化过程中的mRNA稳定性。抗体在鼠脑组织中的定位实验支持其核内分布特征。
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3. **文献名称**: *HNRNPF knockdown impairs cell cycle progression via p53 signaling*
**作者**: Chen, L., Kim, H., et al.
**摘要**: 本研究使用HNRNPF抗体进行RNA干扰后的功能验证，发现HNRNPF缺失导致p53蛋白稳定性增加，从而阻滞结肠癌细胞G1/S期转换。免疫荧光实验证实抗体适用于亚细胞定位研究。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“HNRNPF antibody”或“HNRNPF function”获取最新研究。
The heterogeneous nuclear ribonucleoprotein F (HNRNPF) is a member of the HNRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, transport, stability, and translation regulation. HNRNPF contains three quasi-RRM (qRRM) domains that enable its binding to GU-rich sequences in target RNAs. It participates in alternative splicing decisions, mRNA nuclear export, and miRNA processing, impacting diverse cellular processes such as cell cycle progression, DNA repair, and stress responses.
Antibodies targeting HNRNPF are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). Validated HNRNPF antibodies help elucidate its functional roles in diseases, particularly cancer. For example, HNRNPF is overexpressed in several malignancies (e.g., breast, liver, and colorectal cancers) and correlates with tumor progression, metastasis, and poor prognosis. It also interacts with viral RNAs, influencing viral replication in infections. Additionally, HNRNPF mutations or dysregulation have been linked to neurodevelopmental disorders, including autism spectrum disorders. Commercial HNRNPF antibodies are typically raised in rabbits or mice, with specificity confirmed via knockout validation. Researchers rely on these antibodies to explore HNRNPF's regulatory mechanisms in both physiological and pathological contexts, including its interplay with non-coding RNAs and involvement in therapeutic resistance.
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