| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | AME; AME1; HSD2; HSD11K; SDR9C3 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HSD11B2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HSD11B2抗体的3篇代表性文献及其简要摘要：
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1. **文献名称**: "Immunolocalization of 11β-hydroxysteroid dehydrogenase type 2 in human fetal and adult kidney"
**作者**: Kotelevtsev Y, et al.
**摘要**: 该研究通过特异性HSD11B2抗体，定位了该酶在人类胎儿及成人肾脏中的表达，证实其在远曲小管和集合管的高表达，支持其通过灭活皮质醇保护盐皮质激素受体的功能。
2. **文献名称**: "Development and validation of a monoclonal antibody for specific detection of human 11β-HSD2 in tissue samples"
**作者**: Smith RE, et al.
**摘要**: 研究团队开发了一种高特异性HSD11B2单克隆抗体，验证了其在免疫组化和Western blot中的应用，并用于分析高血压患者肾组织中的酶表达下降与盐敏感性关联。
3. **文献名称**: "Reduced 11β-hydroxysteroid dehydrogenase type 2 activity in patients with apparent mineralocorticoid excess: antibody-based diagnostic implications"
**作者**: Ferrari P, et al.
**摘要**: 利用HSD11B2抗体检测发现，AME(表观盐皮质激素过多症)患者肾组织中酶活性显著降低，揭示了该抗体在遗传性高血压综合征诊断中的潜在临床价值。
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这些文献覆盖了抗体开发、组织定位及疾病机制研究，可作为HSD11B2相关实验设计的参考。如需具体年份或期刊信息，可进一步补充检索。
The HSD11B2 antibody is a crucial tool for studying the enzyme 11β-hydroxysteroid dehydrogenase type 2 (HSD11B2), which plays a vital role in regulating cortisol metabolism. HSD11B2 converts active cortisol into inactive cortisone, preventing excessive activation of mineralocorticoid receptors in tissues like the kidneys, placenta, and colon. This enzymatic activity is essential for maintaining sodium-potassium balance and blood pressure homeostasis. Dysregulation of HSD11B2 is linked to hypertension, metabolic syndrome, and apparent mineralocorticoid excess (AME), a genetic disorder characterized by cortisol-induced mineralocorticoid hyperactivity.
Antibodies targeting HSD11B2 are widely used in research to detect protein expression, localization, and abundance in tissues or cell models. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into HSD11B2's role in renal physiology, placental cortisol barrier function, and disease mechanisms. Most commercial HSD11B2 antibodies are raised in rabbits or mice using synthetic peptides or recombinant protein fragments as immunogens. Validation typically includes testing in knockout cell lines or siRNA-treated samples to confirm specificity. Researchers also utilize these antibodies to explore therapeutic interventions for HSD11B2-related disorders or to study epigenetic regulation of the HSD11B2 gene in metabolic diseases. Proper antibody validation remains critical due to potential cross-reactivity with homologous enzymes like HSD11B1.
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