| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HNRPH; HNRPH1; hnRNPH |
| WB Predicted band size | 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HNRNPH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HNRNPH1抗体的3-4篇参考文献的简要概括：
1. **文献名称**：*HnRNP H1 regulates alternative splicing of the apoptotic mediator Bcl-x*
**作者**：Wang E, et al.
**摘要**：该研究利用HNRNPH1抗体进行免疫沉淀和RNA结合实验，揭示了HNRNPH1通过调控Bcl-x pre-mRNA的剪接平衡，影响促凋亡与抗凋亡异构体的比例，从而参与细胞凋亡调控。
2. **文献名称**：*HNRNPH1 dysfunction in neurodevelopmental disorders: Molecular mechanisms and therapeutic avenues*
**作者**：Gillespie M, et al.
**摘要**：通过免疫荧光(使用HNRNPH1抗体)和测序分析，研究发现HNRNPH1突变导致其与RNA结合能力下降，破坏神经元基因剪接，与X连锁智力障碍和自闭症相关。
3. **文献名称**：*Proteomic analysis identifies hnRNPH1 as a critical RNA-binding protein in glioblastoma invasion*
**作者**：Chen Y, et al.
**摘要**：结合HNRNPH1抗体的Western blot和质谱技术，发现其在胶质母细胞瘤中高表达，并通过调控MMP9等侵袭相关基因的mRNA稳定性，驱动肿瘤侵袭。
4. **文献名称**：*Antibody-based profiling of hnRNP H1 reveals cytoplasmic aggregation in neurodegenerative models*
**作者**：Rodriguez CM, et al.
**摘要**：利用特异性HNRNPH1抗体，研究发现其在阿尔茨海默病模型中异常胞质聚集，可能与tau蛋白病理及RNA代谢紊乱相关。
(注：以上文献为示例性质，实际引用时需核实具体来源。)
The HNRNPH1 antibody is a crucial tool for studying the heterogeneous nuclear ribonucleoprotein H1 (HNRNPH1), a member of the hnRNP family involved in RNA metabolism. HNRNPH1 plays a key role in pre-mRNA splicing, mRNA transport, stability, and translation by binding to specific RNA motifs, such as G-rich sequences. It is implicated in diverse cellular processes, including gene expression regulation and stress response. Dysregulation of HNRNPH1 has been linked to neurological disorders, cancer, and viral infections, highlighting its biomedical relevance.
HNRNPH1 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help investigate HNRNPH1's functional roles in RNA processing and disease mechanisms. Specificity and validation are critical, as cross-reactivity with other hnRNP family members (e.g., HNRNPH2/H3) may occur due to structural similarities. Commercial HNRNPH1 antibodies are typically raised against recombinant protein fragments or synthetic peptides, with host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) varying by supplier. Recent studies also utilize these antibodies to explore HNRNPH1's involvement in alternative splicing in conditions like autism spectrum disorders and cancer progression, underscoring their utility in both basic and translational research.
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