| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | MIP; TRN; IPO2; MIP1; KPNB2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human TNPO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TNPO1抗体的3-4篇参考文献的简要列举(文献信息为虚构示例，仅供参考)：
1. **文献名称**：*TNPO1 facilitates HIV-1 nuclear import by interacting with viral integrase*
**作者**：Christophe Goujon et al.
**摘要**：研究利用TNPO1特异性抗体验证了Transportin-1(TNPO1)在HIV-1病毒整合酶介导的核转运机制中的作用，发现其缺失会显著抑制病毒基因组进入细胞核。
2. **文献名称**：*Role of Transportin-1 in mediating TDP-43 proteinopathy in ALS*
**作者**：Dorothee Dormann & Christian Haass
**摘要**：通过TNPO1抗体进行免疫共沉淀实验，揭示了TNPO1与肌萎缩侧索硬化症(ALS)相关蛋白TDP-43的异常胞质聚集存在关联，提示其在神经退行性疾病中的潜在调控机制。
3. **文献名称**：*TNPO1 as a biomarker in triple-negative breast cancer progression*
**作者**：Maria Schneider et al.
**摘要**：研究利用TNPO1抗体进行组织染色，发现TNPO1在三阴性乳腺癌中高表达，其表达水平与肿瘤转移和患者预后不良显著相关。
4. **文献名称**：*Development and validation of a TNPO1-specific monoclonal antibody for nuclear transport studies*
**作者**：Ulrike Kutay & Harald Fried
**摘要**：报道了一种高特异性TNPO1单克隆抗体的开发，并通过免疫荧光和Western blot验证其在多种细胞模型中研究核转运机制的应用价值。
注：以上文献为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索确认。如需具体文献，建议补充关键词(如研究领域或年份)进一步筛选。
TNPO1 (Transportin-1), also known as Importin-β2. is a member of the karyopherin-β family responsible for nucleocytoplasmic transport. It facilitates nuclear import of specific cargo proteins, particularly mRNA-binding proteins like SR proteins and heterogeneous nuclear ribonucleoproteins (hnRNPs), by recognizing their proline-tyrosine nuclear localization signals (PY-NLS). TNPO1 plays critical roles in cellular processes such as gene expression regulation, RNA processing, and cell cycle control.
TNPO1 antibodies are essential tools for studying its expression, localization, and interactions in both physiological and pathological contexts. Research highlights its involvement in HIV-1 replication, as TNPO1 aids viral integration by transporting HIV-1 capsid proteins into the nucleus. Additionally, TNPO1 dysfunction is linked to neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), where impaired transport of RNA-binding proteins like TDP-43 may contribute to cytoplasmic aggregation.
These antibodies are widely used in techniques like Western blot, immunofluorescence, and immunoprecipitation to investigate TNPO1's role in viral pathogenesis, cancer, and neurodegeneration. Commercial TNPO1 antibodies are typically validated for specificity against human, mouse, or rat samples, aiding mechanistic studies and therapeutic target exploration.
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