| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | AGD3; OCC1; OCC-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human C12orf75 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于C12orf75抗体的假设性参考文献示例(内容基于合理推测，非真实文献)：
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1. **文献名称**: *"Characterization of C12orf75 protein expression in human tissues using a novel monoclonal antibody"*
**作者**: Müller, A. et al.
**摘要**: 本研究开发了一种针对C12orf75的新型小鼠单克隆抗体，通过免疫组化和Western blot分析，发现该蛋白在心脏和骨骼肌中高表达，提示其可能参与能量代谢调控。
2. **文献名称**: *"C12orf75 interacts with mitochondrial complexes and regulates oxidative phosphorylation"*
**作者**: Tanaka, K. & Chen, L.
**摘要**: 利用C12orf75特异性抗体进行免疫共沉淀和质谱分析，发现其与线粒体电子传递链复合物存在物理相互作用，敲低该基因导致细胞ATP合成显著下降。
3. **文献名称**: *"Aberrant C12orf75 expression in glioblastoma correlates with patient survival"*
**作者**: Park, J. et al.
**摘要**: 通过抗体验证发现，C12orf75在胶质母细胞瘤中表达异常升高，且与患者总生存期呈负相关，提示其作为潜在肿瘤标志物的可能性。
4. **文献名称**: *"Developmental regulation of C12orf75 in mouse embryogenesis"*
**作者**: Gupta, R. et al.
**摘要**: 使用兔多克隆抗体进行免疫荧光染色，揭示C12orf75在小鼠胚胎神经管和肢芽发育中动态表达，提示其在神经和肢体形成中的作用。
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注：以上文献为模拟示例，实际研究中建议通过PubMed或Google Scholar以“C12orf75 antibody”为关键词检索最新文献。如需真实文献，可补充基因别名(如PTH2R等)扩大检索范围。
The C12orf75 antibody is a research tool designed to detect the protein encoded by the *C12orf75* gene (Chromosome 12 Open Reading Frame 75), also known as *CABLES2*. This gene is conserved across vertebrates, but its precise biological function remains incompletely characterized. Studies suggest C12orf75 may play roles in cellular processes such as apoptosis, cell cycle regulation, or signal transduction, with potential links to cancer and neurological disorders. The protein is predicted to localize to the cytoplasm and nucleus, though its expression patterns vary across tissues.
C12orf75 antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study protein expression, subcellular localization, and interactions. Commercial antibodies are typically raised in hosts like rabbits or mice, using immunogens derived from synthetic peptides or recombinant protein fragments. Validation often includes knockout cell line controls to confirm specificity.
Research interest in C12orf75 has grown due to its reported dysregulation in diseases. For example, altered expression has been observed in certain cancers, suggesting potential diagnostic or therapeutic relevance. However, functional studies remain limited, and further investigation is needed to clarify its molecular mechanisms. The availability of C12orf75 antibodies supports ongoing efforts to explore its role in health and disease, bridging gaps in understanding this understudied gene.
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