| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ARTD16; pART16 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PARP8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PARP8抗体的示例参考文献(注：以下为假设性示例，实际文献需通过学术数据库验证)：
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1. **"Development and Validation of a Novel PARP8-Specific Antibody for Functional Studies"**
*Authors: Smith A, et al. (2020)*
**摘要**: 本研究开发了一种高特异性PARP8多克隆抗体，并验证了其在Western blot和免疫荧光中的应用。结果显示PARP8在细胞核内富集，且在氧化应激条件下表达上调，提示其可能参与DNA损伤应答。
2. **"PARP8 Regulates Cell Cycle Progression via Interaction with Cyclin-Dependent Kinases"**
*Authors: Johnson B, et al. (2018)*
**摘要**: 通过使用特异性PARP8抗体进行免疫共沉淀，研究发现PARP8与CDK1/2结合，调控G1/S期转换。敲低PARP8导致细胞周期停滞，表明其在增殖中的关键作用。
3. **"Tissue-Specific Expression Profiling of PARP8 Using Monoclonal Antibodies"**
*Authors: Lee C, et al. (2015)*
**摘要**: 利用新开发的小鼠抗PARP8单克隆抗体，分析其在小鼠组织中的分布，发现PARP8在肝脏和睾丸中高表达，提示其可能在代谢或生殖功能中发挥作用。
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**建议**：实际文献可通过PubMed、Google Scholar等平台，以“PARP8 antibody”或“PARP8 function”为关键词检索，并筛选涉及抗体开发或应用的论文。部分研究可能未在摘要中明确提及抗体，需结合全文内容确认。
PARP8 (Poly(ADP-ribose) polymerase 8) is a member of the PARP enzyme family, which catalyzes the transfer of ADP-ribose units to target proteins, influencing processes like DNA repair, chromatin remodeling, and stress response. Unlike well-characterized PARPs (e.g., PARP1/2), PARP8 remains understudied, though it shares the conserved catalytic PARP domain. Emerging evidence suggests PARP8 localizes to the cytoplasm and nucleus, potentially regulating transcription, cell cycle progression, and mitochondrial function. Its expression is tissue-specific, with higher levels observed in the brain, liver, and testes.
PARP8 antibodies are critical tools for investigating its expression, localization, and molecular interactions. These antibodies, often raised against unique epitopes in its N-terminal or catalytic domains, enable applications such as Western blotting, immunofluorescence, and immunoprecipitation. Researchers use them to study PARP8's role in diseases, including cancer, where dysregulated PARP8 expression has been linked to tumor progression and therapy resistance. For instance, PARP8 overexpression in glioblastoma or hepatocellular carcinoma correlates with poor prognosis, suggesting its potential as a therapeutic target.
Challenges in PARP8 research include antibody specificity due to homology with other PARP family members. Recent studies highlight PARP8's distinct substrate preferences and its possible crosstalk with PARP inhibitors like olaparib, though its exact mechanistic contributions remain unclear. Validating PARP8 antibodies with knockout controls is essential to ensure reliability. Ongoing work aims to clarify PARP8's physiological and pathological functions, leveraging these antibodies to explore its interplay with cellular stress pathways and therapeutic potential.
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