| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | VPREB1; IGI; IGVPB; VPREB |
| Entrez GeneID | 7441 |
| clone | 5F8F3 |
| WB Predicted band size | 16.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD179A (AA: extra 20-145) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于RPS6KB2抗体的3篇参考文献示例(虚构内容，供参考)：
1. **文献名称**：*RPS6KB2 regulates cell proliferation via mTORC1 signaling in cancer*
**作者**：Smith A, et al.
**摘要**：研究通过RPS6KB2抗体验证其在多种癌细胞系中的表达，发现其磷酸化水平与mTORC1活性相关，并促进肿瘤细胞增殖。
2. **文献名称**：*Antibody-based profiling of S6 kinase isoforms in human tissues*
**作者**：Lee J, et al.
**摘要**：利用特异性RPS6KB2抗体分析不同组织中的蛋白表达，发现其在肌肉和肝脏中高表达，并与代谢调控相关。
3. **文献名称**：*Role of RPS6KB2 in chemotherapy resistance*
**作者**：Chen H, et al.
**摘要**：通过免疫组化(使用RPS6KB2抗体)发现，该蛋白在卵巢癌耐药患者中过表达，提示其可能作为治疗靶点。
(注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等数据库检索确认。)
The RPS6KB2 antibody is a research tool designed to detect ribosomal protein S6 kinase beta-2 (RPS6KB2), a serine/threonine kinase involved in cell growth, proliferation, and metabolism. RPS6KB2. also known as S6K2. is a downstream effector of the mTORC1 (mechanistic target of rapamycin complex 1) signaling pathway. It shares structural and functional similarities with its isoform RPS6KB1 (S6K1) but exhibits distinct tissue-specific expression patterns, with higher abundance in skeletal muscle and certain cancer types. Upon activation by phosphorylation (e.g., at Thr388), RPS6KB2 regulates protein synthesis by phosphorylating ribosomal proteins and translation factors. Dysregulation of RPS6KB2 has been implicated in diseases such as cancer, diabetes, and obesity, making it a potential therapeutic target.
RPS6KB2 antibodies are widely used in biomedical research to study protein expression, localization, and activation status in cell lines, tissues, or animal models. They are critical for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specific antibodies targeting phosphorylated RPS6KB2 help assess its kinase activity under different experimental conditions, such as mTOR inhibitor treatments. Validation of these antibodies includes testing for cross-reactivity with RPS6KB1 and confirming target specificity using knockout controls. Their applications span cancer biology studies, metabolic disorder research, and investigations into mTOR pathway dynamics.
×
