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产品描述：人二氢嘧啶酶样3(DPYSL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DPYSL3含量。DPYSL3即二氢嘧啶酶样蛋白3，它参与神经系统发育等过程，在轴突生长、神经导向和神经元迁移中起作用。研究显示其表达异常与肿瘤等疾病相关，机制可能涉及调控细胞增殖、迁移和侵袭等信号通路，是潜在的疾病治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于神经生物学研究、肿瘤微环境分析、药物靶点筛选等科研场景，为探索DPYSL3在细胞信号传导、病理模型建立及分子互作机制中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collapsin response mediator protein 4 ELISA Kit; Collapsin response mediator protein 4 long ELISA Kit; CRMP 4 ELISA Kit; CRMP-4 ELISA Kit; CRMP4 ELISA Kit; Dihydropyrimidinase like 3 ELISA Kit; Dihydropyrimidinase related protein 3 ELISA Kit; Dihydropyrimidinase-related protein 3 ELISA Kit; DPYL3_HUMAN ELISA Kit; DPYSL3 ELISA Kit; DRP 3 ELISA Kit; DRP-3 ELISA Kit; DRP3 ELISA Kit; LCRMP ELISA Kit; TUC4 ELISA Kit; ULIP 1 ELISA Kit; ULIP ELISA Kit; ULIP-1 ELISA Kit; ULIP1 ELISA Kit; UNc 33 like phosphoprotein 1 ELISA Kit; Unc 33 like phosphoprotein ELISA Kit; Unc-33-like phosphoprotein 1 ELISA Kit
缩写：DPYSL3
Uniprot No.：Q14195
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DPYSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-94 | ||||||
| 1:2 | Average % | 106 | |||||
| Range % | 103-109 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
回收率：
| The recovery of human DPYSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 80-88 | |||||
| EDTA plasma (n=4) | 95 | 91-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.2752.1982.2372.14510001.7721.8021.7871.6955001.3081.3001.3041.2122500.9021.0000.9510.8591250.6450.6560.6510.55962.50.4410.4380.4400.34831.250.3030.3100.3070.21500.0910.0920.092 | |||||||
货期：3-5 working days
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