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产品描述：人表皮生长因子样结构域蛋白7(EGFL7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EGFL7含量。EGFL7 即表皮生长因子样蛋白 7，在血管发育、肿瘤血管生成等过程中发挥重要作用。研究表明，它可调节内皮细胞迁移、增殖和血管管腔形成。其异常表达与肿瘤进展、转移相关，有望成为肿瘤诊断和治疗的新靶点，为肿瘤防治带来新思路。试剂盒检测范围为0.47 μg/mL-30 μg/mL，该产品适用于体外实验研究，例如探究心血管疾病、肿瘤生物学或组织修复过程中EGFL7的动态变化，亦可用于评估不同实验条件下（如药物处理、基因编辑）该蛋白的表达调控机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EGF like domain 7 ELISA Kit; EGF like domain containing protein 7 ELISA Kit; EGF like domain multiple 7 ELISA Kit; EGF-like protein 7 ELISA Kit; EGFL 7 ELISA Kit; EGFL7 ELISA Kit; EGFL7_HUMAN ELISA Kit; Epidermal growth factor like domain protein 7 ELISA Kit; Epidermal growth factor-like protein 7 ELISA Kit; MEGF 7 ELISA Kit; MEGF7 ELISA Kit; MGC111117 ELISA Kit; Multiple EGF like domain protein 7 ELISA Kit; Multiple EGF-like domains protein 7 ELISA Kit; Multiple epidermal growth factor like domain protein 7 ELISA Kit; Multiple epidermal growth factor-like domains protein 7 ELISA Kit; NEU1 ELISA Kit; NEU1 protein ELISA Kit; NOTCH4 like protein ELISA Kit; NOTCH4-like protein ELISA Kit; RP11 251M1.2 ELISA Kit; UNQ187/PRO1449 ELISA Kit; Vascular endothelial statin ELISA Kit; VE statin ELISA Kit; VE-statin ELISA Kit; ZNEU 1 ELISA Kit; ZNEU1 ELISA Kit
缩写：EGFL7
Uniprot No.：Q9UHF1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.47 μg/mL-30 μg/mL
灵敏度：0.12 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EGFL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 83-101 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 82-101 | |||||
回收率：
| The recovery of human EGFL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-98 | ||||
| EDTA plasma (n=4) | 94 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected 302.7532.6182.6862.572 152.4212.4042.4132.299 7.51.8941.8771.8861.772 3.751.1861.1971.1921.078 1.870.6100.6340.6220.508 0.940.4110.4070.4090.295 0.470.2650.2710.2680.154 00.1120.1150.114 | ||||||
货期：3-5 working days
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