首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人皮卡丘素(EGFLAM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EGFLAM含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品为科研领域研究者提供高效工具，支持细胞生物学实验中的蛋白表达动态监测、疾病模型中的分子机制研究，以及生物样本中EGFLAM的定量分析等应用场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Agrin-like protein ELISA Kit; AGRINL ELISA Kit; AGRNL ELISA Kit; EGF-like ELISA Kit; EGF-like; fibronectin type III and laminin G domains ELISA Kit; EGF-like; fibronectin type-III and laminin G-like domain-containing protein ELISA Kit; EGFLA_HUMAN ELISA Kit; EGFLAM ELISA Kit; fibronectin type-III and laminin G-like domain-containing protein ELISA Kit; FLJ39155 ELISA Kit; Pikachurin ELISA Kit
缩写：EGFLAM
Uniprot No.：Q63HQ2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EGFLAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-92 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of human EGFLAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 95-104 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7172.7872.7522.64410002.1202.1602.1402.0325001.4681.4251.4471.3392500.7980.7690.7840.6761250.4690.4570.4630.35562.50.2900.2800.2850.17731.250.2120.2250.2190.11100.1070.1090.108 | ||||||
货期：3-5 working days
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