首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠argonaute-2蛋白(EIF2C2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EIF2C2含量。EIF2C2即真核翻译起始因子2C2，参与RNA干扰途径。研究表明其能与miRNA或siRNA结合形成RISC复合物，沉默靶mRNA，在基因表达调控中发挥关键作用，其功能异常与肿瘤等多种疾病发生发展相关，是潜在治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于研究RNA干扰机制、基因沉默通路调控以及相关疾病模型中AGO2的功能变化，例如神经退行性疾病、肿瘤微环境或干细胞分化等领域的基础科研本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ago2 ELISA Kit; Eif2c2 ELISA Kit; Kiaa4215 ELISA Kit; Protein argonaute-2 ELISA Kit; Argonaute2 ELISA Kit; mAgo2 ELISA Kit; EC 3.1.26.n2 ELISA Kit; Argonaute RISC catalytic component 2 ELISA Kit; Eukaryotic translation initiation factor 2C 2 ELISA Kit; eIF-2C 2 ELISA Kit; eIF2C 2 ELISA Kit; Piwi/argonaute family protein meIF2C2 ELISA Kit; Protein slicer ELISA Kit
缩写：EIF2C2
Uniprot No.：Q8CJG0
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse EIF2C2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 106 | ||||
| Range % | 100-110 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of mouse EIF2C2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-93 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6512.5172.5842.44610002.2732.3562.3152.1775001.8011.7271.7641.6262500.9751.0251.0000.8621250.5450.5790.5620.42462.50.3360.3590.3480.21031.250.2570.2490.2530.11500.1340.1410.138 | ||||||
货期：3-5 working days
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