首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人argonaute-2蛋白(EIF2C2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EIF2C2含量。EIF2C2是一种重要靶点。它参与基因表达的调控，在RNA干扰等过程中发挥关键作用。研究其机制有助于深入理解细胞内基因调控网络，对于探索疾病发生发展机制，如肿瘤等疾病，以及开发新的治疗靶点和策略具有重要意义。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于研究RNA干扰机制、基因表达调控及相关疾病（如癌症、神经退行性疾病）的分子机制探索，为体外实验模型或生物样本中AGO2蛋白表达水平分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ago 2 ELISA Kit; AGO2_HUMAN ELISA Kit; Argonaute 2 ELISA Kit; argonaute 2; RISC catalytic component ELISA Kit; Argonaute RISC catalytic component 2 ELISA Kit; Argonaute2 ELISA Kit; CTA-204B4.6 ELISA Kit; dAgo2 ELISA Kit; eIF 2C 2 ELISA Kit; eIF-2C 2 ELISA Kit; eIF2C 2 ELISA Kit; Eif2c2 ELISA Kit; Eukaryotic translation initiation factor 2C 2 ELISA Kit; Eukaryotic translation initiation factor 2C subunit 2 ELISA Kit; hAgo2 ELISA Kit; MGC3183 ELISA Kit; PAZ Piwi domain protein ELISA Kit; PPD ELISA Kit; Protein argonaute-2 ELISA Kit; Protein slicer ELISA Kit; Q10 ELISA Kit; Slicer protein ELISA Kit
缩写：EIF2C2
Uniprot No.：Q9UKV8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EIF2C2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of human EIF2C2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-105 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.1972.3252.2612.1158001.3871.4861.4371.2914000.8330.7990.8160.6702000.4950.5070.5010.3551000.3040.3160.3100.164500.2420.2310.2370.091250.1880.1840.1860.04000.1450.1460.146 | ||||||
货期：3-5 working days
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