Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 2(ENPP2) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL007680MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠外核苷酸焦磷酸酶/磷酸二酯酶家族成员2(ENPP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENPP2含量。ENPP2，又称自分泌运动因子（Autotaxin），是一种分泌的溶血磷脂酶D，作用于循环的溶血磷脂酰胆碱，产生溶血磷脂酸（LPA）。其在肿瘤细胞增殖、迁移和骨代谢中发挥重要作用，是近年来研究的热点。试剂盒检测范围为72 ng/mL-2500 ng/mL，科研人员可将其应用于疾病动物模型中ENPP2的动态表达研究，例如探索其在代谢紊乱、组织纤维化或肿瘤进展中的分子机制，也可用于评估药物干预或基因编辑后生物标志物的水平变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Enpp2 ELISA Kit; Npps2 ELISA Kit; Pdnp2 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 ELISA Kit; E-NPP 2 ELISA Kit; EC 3.1.4.39 ELISA Kit; Autotaxin ELISA Kit; Extracellular lysophospholipase D ELISA Kit; LysoPLD ELISA Kit
缩写：
ENPP2
Uniprot No.：
Q9R1E6
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
72 ng/mL-2500 ng/mL
灵敏度：
36 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	87
	Range %	81-94
1:4	Average %	98
	Range %	92-102
1:8	Average %	94
	Range %	87-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
2500	1.735	1.687	1.711	1.663
893	0.835	0.773	0.804	0.756
357	0.392	0.389	0.391	0.343
179	0.245	0.232	0.239	0.191
72	0.121	0.127	0.124	0.076
0	0.048	0.047	0.048