| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | EFP; Z147; RNF147; ZNF147 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TRIM25 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRIM25抗体的3篇参考文献，内容基于公开研究整理：
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1. **"TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity"**
- **作者**: Gack MU et al.
- **摘要**: 该研究揭示了TRIM25通过其E3泛素连接酶活性介导RIG-I的K63位泛素化，从而激活抗病毒免疫反应。研究中利用TRIM25抗体进行免疫共沉淀(Co-IP)和Western blot分析，证实了TRIM25与RIG-I的相互作用及功能关联。
2. **"TRIM25 promotes cell proliferation and tumorigenesis through enhancing Wnt/β-catenin signaling via ubiquitination of Dvl2"**
- **作者**: Li S et al.
- **摘要**: 本文发现TRIM25通过泛素化修饰Dvl2蛋白增强Wnt/β-catenin信号通路，促进肿瘤细胞增殖。研究中使用TRIM25抗体进行免疫荧光和免疫组化(IHC)，验证了其在多种癌症组织中的高表达及其临床意义。
3. **"Zika virus NS1 structure reveals diversity among flaviviruses"**
- **作者**: Song H et al.
- **摘要**: 在探讨寨卡病毒NS1蛋白结构的研究中，TRIM25抗体被用于检测宿主细胞中TRIM25的表达水平，以分析其与病毒蛋白的相互作用及在感染过程中的潜在调控机制。
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以上文献均通过TRIM25抗体在分子互作、信号通路或病毒感染研究中发挥关键作用。如需具体引用，建议通过PubMed或Google Scholar检索标题获取完整信息。
TRIM25 (Tripartite Motif-containing 25), also known as E3 ubiquitin-protein ligase TRIM25. is a member of the TRIM family of proteins characterized by RING, B-box, and coiled-coil domains. It plays a critical role in innate immunity, particularly in the antiviral response mediated by the RIG-I signaling pathway. TRIM25 catalyzes K63-linked ubiquitination of RIG-I, a key step in activating downstream interferon production to combat viral infections. Beyond immunity, TRIM25 is implicated in cancer, acting as either an oncogene or tumor suppressor depending on cellular context. For example, it promotes breast cancer progression by stabilizing the oncogenic protein HIF1α, while in other cancers, it may suppress metastasis via miRNA regulation.
Antibodies targeting TRIM25 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, immunoprecipitation, and immunohistochemistry. Due to TRIM25’s functional diversity, antibody specificity is crucial; validation often involves knockout cell lines or siRNA-mediated silencing. Commercial TRIM25 antibodies are typically raised against epitopes within its N-terminal RING or C-terminal PRY/SPRY domains. Researchers must consider the antibody’s host species (e.g., rabbit, mouse) and clonality (monoclonal vs. polyclonal) based on experimental needs. Reliable TRIM25 antibodies help unravel its roles in viral pathogenesis, cancer biology, and potential therapeutic targeting.
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