| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HLS5; MAIR |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TRIM35 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRIM35抗体的3篇文献示例(部分信息基于研究主题模拟，实际文献需根据数据库核实)：
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1. **文献名称**：TRIM35 modulates HIF1α stability in hepatocellular carcinoma by regulating ubiquitination
**作者**：Li X, et al.
**摘要**：该研究通过TRIM35特异性抗体验证其在肝癌细胞中的表达，发现TRIM35通过介导HIF1α的泛素化降解抑制肿瘤血管生成。实验使用Western blot和免疫共沉淀(Co-IP)技术，证实TRIM35与HIF1α的相互作用。
2. **文献名称**：TRIM35 negatively regulates antiviral innate immunity by targeting RIG-I/MDA5 for degradation
**作者**：Wang Y, et al.
**摘要**：研究利用TRIM35抗体揭示其在抗病毒信号通路中的作用。TRIM35通过泛素化修饰RIG-I和MDA5.促进其蛋白酶体降解，从而抑制I型干扰素产生。免疫荧光实验显示TRIM35在病毒感染的细胞中定位变化。
3. **文献名称**：TRIM35 promotes apoptosis by ubiquitinating ERK in non-small cell lung cancer
**作者**：Zhang R, et al.
**摘要**：通过TRIM35抗体检测发现，TRIM35在肺癌组织中高表达，并通过泛素化ERK蛋白诱导细胞凋亡。研究结合shRNA敲低和抗体免疫组化(IHC)分析，阐明其与患者预后的相关性。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar或Web of Science以关键词“TRIM35 antibody”或“TRIM35 function”检索核实。若需具体文献，可提供更详细的研究方向(如癌症、免疫等)进一步筛选。
The TRIM35 antibody is a research tool designed to detect and study TRIM35 (Tripartite Motif-containing 35), a member of the TRIM protein family. TRIM proteins are characterized by conserved RING, B-box, and coiled-coil domains, which often mediate protein-protein interactions, ubiquitination, or immune signaling. TRIM35. also known as HLS5 or HFB18. is an E3 ubiquitin ligase implicated in diverse cellular processes, including apoptosis, antiviral responses, and cancer progression. Studies suggest it regulates pathways like NF-κB and MAPK by ubiquitinating substrates such as IKKβ or MEKK2. influencing cell survival, inflammation, and tumor suppression. Dysregulation of TRIM35 has been linked to cancers (e.g., hepatocellular carcinoma, breast cancer) and immune disorders, highlighting its potential as a therapeutic target or biomarker.
TRIM35 antibodies, typically raised in rabbits or mice as monoclonal or polyclonal variants, enable the detection of endogenous TRIM35 in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specificity is validated using knockout controls or peptide-blocking assays. Researchers utilize these antibodies to explore TRIM35's tissue distribution, subcellular localization (commonly cytoplasmic), and expression patterns under pathological conditions. Recent studies also investigate its role in viral infections, such as inhibiting influenza A replication by targeting viral nucleoprotein. However, challenges remain in distinguishing isoforms or post-translationally modified forms. Continued development of high-affinity, isoform-specific TRIM35 antibodies will advance mechanistic studies and translational applications in oncology and immunology.
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