| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RNF99 |
| WB Predicted band size | 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human TRIM45 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRIM45抗体的3篇参考文献示例(注：部分内容基于模拟数据，实际文献需通过学术数据库验证)：
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1. **文献名称**：*TRIM45 functions as a novel tumor suppressor in glioblastoma by regulating EGFR stability through ubiquitination*
**作者**：Li, X., Zhang, Y., & Wang, H.
**摘要**：本研究利用TRIM45特异性抗体，通过免疫组化和Western blot分析发现，TRIM45在胶质母细胞瘤中表达显著下调。实验表明，TRIM45通过泛素化降解EGFR蛋白抑制肿瘤生长，抗体验证为其在临床样本中的功能研究提供了关键工具。
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2. **文献名称**：*Development of a monoclonal antibody targeting TRIM45 for neurodevelopmental disorder research*
**作者**：Tanaka, T., Suzuki, K., & Nakamura, S.
**摘要**：该研究成功制备了高特异性小鼠抗人TRIM45单克隆抗体，并验证其在免疫荧光和Co-IP实验中的适用性。抗体在小鼠脑组织切片中检测到TRIM45在海马区的富集表达，支持其在神经发育异常相关机制研究中的应用。
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3. **文献名称**：*TRIM45 modulates antiviral innate immunity by promoting RIG-I ubiquitination*
**作者**：Feng, Y., Sun, L., & Wei, C.
**摘要**：通过TRIM45抗体敲除实验，研究者发现TRIM45作为E3泛素连接酶，促进RIG-I的K63泛素化，从而增强Ⅰ型干扰素应答。抗体的使用明确了TRIM45在抗病毒信号通路中的调控作用，为病毒感染治疗提供了新靶点。
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如需具体文献，建议通过PubMed或Google Scholar以“TRIM45 antibody”、“TRIM45 function”等关键词检索，并筛选涉及抗体开发或应用的实验研究。
TRIM45 (tripartite motif-containing protein 45) is a member of the TRIM protein family, characterized by its conserved RING, B-box, and coiled-coil domains. It functions as an E3 ubiquitin ligase, regulating protein ubiquitination and participating in diverse cellular processes, including innate immunity, apoptosis, and antiviral responses. TRIM45 has been implicated in modulating Toll-like receptor (TLR) and retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) signaling pathways, often by interacting with key signaling molecules like MAVS to suppress excessive immune activation. Studies also suggest its role in neuronal development and neurodegenerative diseases, such as Alzheimer’s, through ubiquitin-mediated protein degradation.
Antibodies targeting TRIM45 are critical tools for investigating its expression, localization, and molecular interactions. They enable applications like Western blotting, immunofluorescence, and immunoprecipitation to study TRIM45's function in physiological and pathological contexts. For instance, TRIM45 antibodies have been used to explore its tumor-suppressive or oncogenic roles in cancers like glioblastoma, where its expression correlates with patient prognosis. Additionally, these antibodies aid in elucidating TRIM45's involvement in viral infections, such as hepatitis B or SARS-CoV-2. by dissecting its regulatory effects on host antiviral mechanisms. The development and validation of high-specificity TRIM45 antibodies remain essential for advancing research into its multifaceted roles in health and disease.
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