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产品描述：人外核苷三磷酸二磷酸水解酶1(ENTPD1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENTPD1含量。ENTPD1是一种质膜蛋白，编码的蛋白质能将细胞外ATP和ADP水解为AMP，可能对血小板聚集有抑制作用，与癌症和免疫调节有关。研究发现，ENTPD1与精神分裂症的发病机制密切相关，可能成为潜在的血液生物标志物。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于体外研究ENTPD1在免疫疾病、癌症或代谢性疾病中的表达特征及功能机制。可为科研人员探索ENTPD1在细胞信号转导、免疫细胞功能调控等领域的分子作用提供有效工具，广泛应用于基础医学、药理学及生物标志物筛选等研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATPDase ELISA Kit; CD 39 ELISA Kit; CD39 ELISA Kit; CD39 antigen ELISA Kit; DKFZp686D194 ELISA Kit; DKFZp686I093 ELISA Kit; Ecto apyrase ELISA Kit; Ecto ATP diphosphohydrolase ELISA Kit; Ecto-apyrase ELISA Kit; Ecto-ATP diphosphohydrolase 1 ELISA Kit; Ecto-ATPase 1 ELISA Kit; Ecto-ATPDase 1 ELISA Kit; Ectonucleoside triphosphate diphosphohydrolase 1 ELISA Kit; ENTP1_HUMAN ELISA Kit; ENTPD 1 ELISA Kit; ENTPD1 ELISA Kit; FLJ40921 ELISA Kit; FLJ40959 ELISA Kit; Lymphoid cell activation antigen ELISA Kit; NTPDase 1 ELISA Kit; NTPDase1 ELISA Kit; SPG64 ELISA Kit
缩写：ENTPD1
Uniprot No.：P49961
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ENTPD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-102 | |||||
回收率：
| The recovery of human ENTPD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 97-103 | ||||
| EDTA plasma (n=4) | 107 | 103-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.1612.3432.2522.0597501.7241.7081.7161.5233751.2961.2741.2851.092187.50.8910.8850.8880.69593.750.5620.5980.5800.38746.880.4010.4210.4110.21823.440.2870.2890.2880.09500.1910.1940.193 | ||||||
货期：3-5 working days
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