Rabbit Polyclonal WASF2 Antibody

The WASF2 antibody is a crucial tool for studying the WASF2 protein (also known as WAVE2), a member of the Wiskott-Aldrich syndrome protein (WASP) family. WASF2 regulates actin cytoskeleton reorganization by interacting with the ARP2/3 complex, a process essential for cell migration, invasion, and membrane protrusion dynamics. Its activity is tightly controlled through Rac1 GTPase signaling, which releases WASF2 from a multi-protein inhibitory complex (including CYFIP and ABI1) to promote actin branching. Dysregulation of WASF2 is linked to cancer metastasis, particularly in breast, colorectal, and prostate cancers, where its overexpression enhances tumor cell invasiveness by driving epithelial-mesenchymal transition (EMT). Researchers use WASF2 antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to quantify protein levels, assess subcellular localization, and correlate expression with clinical outcomes. These antibodies also aid in functional studies, such as knockdown experiments to explore WASF2's role in metastatic pathways. Validating antibody specificity is critical, as cross-reactivity with other WASP family members (e.g., WASF1/WAVE1) could skew data interpretation. Overall, WASF2 antibodies are pivotal in advancing our understanding of cytoskeletal dynamics in both physiological and pathological contexts.