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Mouse Epinephrine/Adrenaline,EPI ELISA Kit

  • 中文名: 小鼠肾上腺素(EPI)ELISA Kit
货号: IPD-E08679m
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠肾上腺素(EPI)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的EPI含量。小鼠肾上腺素(EPI)是肾上腺分泌的一种激素,对心血管、代谢和应激反应等生理过程至关重要。研究其作用机制,有助于揭示相关疾病如心血管疾病和代谢综合征的发病机制,并为疾病治疗提供新思路。试剂盒检测范围为0.78 ng/ml-50 ng/ml,适用于多种科研场景,包括应激生理学模型中激素动态变化的监测、药物或环境因素对交感 - 肾上腺轴影响的评估,以及代谢性疾病或心血管研究中EPI调控机制的探索。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    EPI
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates.
  • 检测范围:
    0.78 ng/ml-50 ng/ml.
  • 灵敏度:
    0.2 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    84


    Range %

    80-92


    1:2

    Average %

    87


    Range %

    85-100


    1:4

    Average %

    85


    Range %

    82-99


    1:8

    Average %

    89


    Range %

    86-98


  • 回收率:

    The recovery of mouse EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    85

    82-90


    EDTA plasma (n=4)

    87

    83-96


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average




    50

    0.180

    0.174

    0.177




    25

    0.307

    0.317

    0.312




    12.5

    0.485

    0.508

    0.496




    6.25

    0.750

    0.729

    0.740




    3.12

    1.228

    1.301

    1.265




    1.56

    1.972

    1.953

    1.963




    0.78

    2.565

    2.484

    2.524




    0

    2.916

    2.825

    2.871





  • 货期:
    3-5 working days

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