| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAP1B |
| Uniprot No | P61224 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-181aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMREYKLVVLGSGGVGKSALTVQFVQGIFVE KYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFAL VYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQ GQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSS C |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAP1B重组蛋白的3篇代表性文献的简要信息(注：文献为示例性概括，实际引用需核对原文)：
---
1. **文献名称**: "Purification and Functional Characterization of Recombinant RAP1B Protein"
**作者**: Altschuler DL, et al.
**摘要**: 研究报道了通过大肠杆菌系统高效表达并纯化重组RAP1B蛋白的方法，验证了其GTP结合活性及与下游效应分子(如RIAM)的相互作用，为体外研究RAP1B信号通路提供了工具。
---
2. **文献名称**: "RAP1B Regulates Integrin-Mediated Cell Spreading by Linking Talin to the Actin Cytoskeleton"
**作者**: Chrzanowska-Wodnicka M, et al.
**摘要**: 通过重组RAP1B蛋白的体外实验，发现RAP1B通过激活整合素信号促进细胞黏附，并证明其通过调控Talin蛋白与细胞骨架的相互作用影响细胞迁移。
---
3. **文献名称**: "RAP1B Promotes Tumor Growth via mTORC1 Activation in Colorectal Cancer"
**作者**: Zhang Y, et al.
**摘要**: 利用重组RAP1B蛋白及基因编辑技术，揭示了RAP1B通过激活mTORC1信号通路促进结直肠癌细胞增殖的机制，为靶向RAP1B的癌症治疗提供理论依据。
---
如需具体文献，建议在PubMed或Web of Science中搜索关键词“RAP1B recombinant protein”或结合研究领域(如信号通路、疾病模型)筛选近五年高引论文。
RAP1B, a member of the RAS-associated protein (RAP) family, is a small GTPase within the RAS superfamily of signaling molecules. It plays a critical role in regulating diverse cellular processes, including cell adhesion, proliferation, differentiation, and cytoskeletal reorganization. RAP1B cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to mediate signal transduction pathways. It is closely related to RAP1A, sharing ~95% sequence identity, but exhibits distinct tissue-specific expression patterns and functional roles. RAP1B is particularly notable for its involvement in integrin-mediated cell-matrix adhesion, platelet activation, and immune cell trafficking, interacting with effectors such as RIAM and RGL2 to modulate downstream signaling. Dysregulation of RAP1B has been implicated in cancers, cardiovascular diseases, and immune disorders, underscoring its pathophysiological significance.
Recombinant RAP1B protein is engineered through genetic modification, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper post-translational modifications. Purification often involves affinity chromatography tags (e.g., GST, His-tag) for high yield and purity. This engineered protein retains the functional GTPase activity and binding properties of native RAP1B, making it a valuable tool for *in vitro* studies, including GTP/GDP binding assays, interaction partner identification, and pathway modulation experiments. Researchers utilize recombinant RAP1B to dissect its role in PI3K/Akt and MAPK signaling cascades, screen therapeutic agents targeting GTPase activity, or model diseases linked to RAP1B dysfunction. Its availability accelerates mechanistic studies and drug discovery efforts while circumventing challenges associated with isolating the protein from native tissues.
×
