| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAP2A |
| Uniprot No | P10114 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMREYKVVVLGSGGVGKSALTVQFVTGTFIE KYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFIL VYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSSE GRALAEEWGCPFMETSAKSKTMVDELFAEIVRQMNYAAQPDKDDPCCSAC |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAP2A重组蛋白的模拟参考文献示例(注：以下内容为虚构，仅用于演示格式)：
1. **文献名称**: "Recombinant RAP2A Protein Expression and GTPase Activity Analysis in E. coli"
**作者**: Zhang, L. et al.
**摘要**: 本研究成功构建了人源RAP2A的重组表达载体，在大肠杆菌系统中实现高效可溶性表达，并通过镍柱亲和层析纯化获得高纯度蛋白。体外GTP水解实验表明，重组RAP2A具有依赖于Mg²⁺的GTP酶活性，为后续功能研究奠定基础。
2. **文献名称**: "Crystal Structure of RAP2A in the GDP-Bound State Reveals Conformational Switch Mechanism"
**作者**: Chen, Y. & Wang, H.
**摘要**: 通过X射线晶体学解析了RAP2A与GDP结合状态的三维结构(分辨率2.1 Å)，发现其Switch I/II区域构象变化显著。该研究揭示了RAP2A与下游效应分子相互作用的潜在位点，为靶向调控提供结构依据。
3. **文献名称**: "RAP2A Regulates Wnt/β-Catenin Signaling via Recombinant Protein-Mediated Pathway Reconstitution"
**作者**: Li, X. et al.
**摘要**: 利用重组RAP2A蛋白在HEK293细胞模型中证明，RAP2A通过结合Dishevelled蛋白激活Wnt/β-catenin信号通路，荧光素酶报告基因检测显示其过表达可显著增强通路活性，提示其在胚胎发育和肿瘤中的作用。
4. **文献名称**: "RAP2A Recombinant Protein Suppresses Autophagy in Cancer Cells by mTORC1 Activation"
**作者**: Gupta, S. et al.
**摘要**: 研究发现，外源性重组RAP2A蛋白通过激活mTORC1通路抑制乳腺癌细胞自噬，Western blot显示LC3-II水平下降。该效应依赖RAP2A的GTP结合能力，为癌症治疗提供新靶点。
(注：以上文献信息为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索。)
RAP2A (Ras-related protein Rap-2A) is a small GTPase belonging to the Ras superfamily, specifically the RAP subfamily, which includes RAP1 and RAP2 isoforms. It plays a pivotal role in intracellular signaling pathways regulating cell proliferation, differentiation, migration, and cytoskeletal organization. Structurally, RAP2A contains conserved GTP-binding domains and effector interaction regions, enabling its function as a molecular switch cycling between active GTP-bound and inactive GDP-bound states. Its activity is tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).
RAP2A is implicated in diverse physiological processes, including vesicle trafficking, cell adhesion, and synaptic plasticity. Recent studies highlight its involvement in the Hippo-YAP signaling pathway, where it modulates organ size control and tissue regeneration. Dysregulation of RAP2A has been linked to pathological conditions such as cancer metastasis, cardiovascular diseases, and neurological disorders. In cancer, aberrant RAP2A expression correlates with tumor invasiveness and drug resistance, making it a potential therapeutic target.
Recombinant RAP2A protein is typically produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems to ensure proper post-translational modifications. The purified protein retains GTPase activity and structural integrity, serving as a critical tool for *in vitro* studies, including protein interaction assays, enzymatic activity measurements, and drug screening. Its applications extend to structural biology for elucidating GTPase regulatory mechanisms and developing targeted inhibitors. Researchers frequently employ tags (e.g., His-tag, GST) for efficient purification and detection. As interest grows in precision medicine, recombinant RAP2A continues to facilitate mechanistic insights into cellular signaling networks and biomarker discovery.
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