| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAP2B |
| Uniprot No | P61225 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-183aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMREYKVVVLGSGGVGKSALTVQFVTGS FIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQG FILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVS YGEGKALAEEWSCPFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCC SACVIL |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RAP2B重组蛋白的参考文献示例(注：文献信息为假设性概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *"Cloning and Functional Characterization of Recombinant Human RAP2B Protein"*
**作者**: Zhang Y. et al.
**摘要**: 本研究成功克隆了人源RAP2B基因，并在大肠杆菌中高效表达重组RAP2B蛋白。通过亲和层析纯化获得高纯度蛋白，并验证其GTPase活性。实验表明，重组RAP2B在体外可激活下游MAPK信号通路，提示其在细胞增殖调控中的潜在作用。
2. **文献名称**: *"RAP2B Promotes Tumor Angiogenesis via VEGF Signaling in Colorectal Cancer"*
**作者**: Wang L. et al.
**摘要**: 研究利用重组RAP2B蛋白处理内皮细胞，发现其通过上调VEGF表达促进血管生成。动物模型显示，RAP2B过表达显著增强肿瘤血管密度，提示其作为癌症治疗靶点的潜力。
3. **文献名称**: *"RAP2B Regulates Hippo-YAP Signaling through Interaction with MST1 Kinase"*
**作者**: Chen X. et al.
**摘要**: 通过体外激酶实验(使用重组RAP2B蛋白)，本文揭示RAP2B与Hippo通路核心激酶MST1直接结合，抑制YAP磷酸化，进而促进细胞增殖和组织生长，为器官发育机制提供了新见解。
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**建议**：若需真实文献，可在PubMed或Google Scholar中以关键词“RAP2B recombinant”“RAP2B signaling”检索，并筛选涉及重组蛋白制备、功能验证或机制研究的文章。
RAP2B (Ras-related protein Rap-2b) is a small GTPase belonging to the RAS superfamily, specifically the RAP subfamily. It functions as a molecular switch by cycling between active GTP-bound and inactive GDP-bound states, regulating downstream signaling pathways involved in cell adhesion, proliferation, and cytoskeletal dynamics. Discovered in the 1990s, RAP2B shares structural homology with other RAP proteins (RAP1A, RAP1B, RAP2A) but exhibits distinct functional roles, particularly in cellular responses to mechanical stress and Hippo signaling pathways.
Recombinant RAP2B protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, biologically active forms for research. Its molecular weight is approximately 21 kDa, with conserved GTP-binding domains critical for interaction with effector molecules such as MAP kinases and RASSF proteins. Studies highlight its involvement in pathological processes, including cancer progression (e.g., promoting tumor growth in colorectal and lung cancers) and cardiovascular diseases (e.g., modulating endothelial cell function in hypertension).
Researchers utilize recombinant RAP2B to investigate its regulatory mechanisms, post-translational modifications (e.g., prenylation), and crosstalk with pathways like Wnt/β-catenin or YAP/TAZ. Challenges in production include maintaining protein stability and mimicking native lipid modifications, often addressed through codon optimization or fusion tags. Current applications range from in vitro binding assays to therapeutic target validation, reflecting its emerging role as a biomarker and potential intervention point in precision medicine.
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