首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人成纤维细胞生长因子18(FGF18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FGF18含量。FGF18即成纤维细胞生长因子18，在胚胎发育、组织修复与再生等过程发挥关键作用。研究主要聚焦其信号通路机制，它可与特定受体结合激活下游信号，影响细胞增殖、分化等活动，在骨疾病、肿瘤等疾病治疗领域有潜在应用价值。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，支持骨骼生物学研究、软骨再生机制探索、药物筛选模型中FGF18表达动态监测以及相关疾病的分子机制解析等基础研究领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF 18 ELISA Kit; FGF-18 ELISA Kit; Fgf18 ELISA Kit; FGF18_HUMAN ELISA Kit; FGFI ELISA Kit; Fibroblast growth factor 18 ELISA Kit; zFGF5 ELISA Kit
缩写：FGF18
Uniprot No.：O76093
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 99 | |||||||||||
| Range % | 89-107 | ||||||||||||
| 1:2 | Average % | 92 | |||||||||||
| Range % | 87-96 | ||||||||||||
| 1:4 | Average % | 95 | |||||||||||
| Range % | 90-100 | ||||||||||||
| 1:8 | Average % | 98 | |||||||||||
| Range % | 94-103 | ||||||||||||
回收率：
| The recovery of human FGF18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 102 | 96-108 | |||||||||||
| EDTA plasma (n=4) | 96 | 92-100 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| pg/mlOD1OD2AverageCorrected 20002.3032.2272.2652.113 10001.6851.7651.7251.573 5001.1821.1631.1731.021 2500.8090.8150.8120.660 1250.4660.4540.4600.308 62.50.3440.3340.3390.187 31.250.2200.2280.2240.072 00.1520.1510.152 | |||||||||||||
货期：3-5 working days
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