| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EBN2; BFNC2; KV7.3 |
| WB Predicted band size | 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human KCNQ3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KCNQ3抗体的参考文献示例(部分信息基于研究领域常见内容构建，供参考)：
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1. **文献名称**: "Immunolocalization of the KCNQ3 potassium channel in the rat central nervous system"
**作者**: Cooper EC, et al.
**摘要**: 本研究利用特异性KCNQ3抗体，通过免疫组化技术揭示了KCNQ3通道蛋白在大脑皮层、海马及小脑中的广泛分布，提示其在调节神经元兴奋性中的关键作用。抗体验证显示与KCNQ2存在共定位，支持KCNQ2/KCNQ3异源通道的形成。
2. **文献名称**: "Differential subcellular localization of KCNQ3 and KCNQ2 channels in hippocampal neurons"
**作者**: Schroeder BC, et al.
**摘要**: 通过免疫荧光与共聚焦显微镜，本研究使用KCNQ3特异性抗体证明了KCNQ3在神经元胞体和树突的富集，与KCNQ2共同调控突触后M电流。抗体特异性经敲除小鼠模型验证，排除了交叉反应性。
3. **文献名称**: "Antibody-based profiling of KCNQ3 expression in developmental and epileptic encephalopathies"
**作者**: Maljevic S, et al.
**摘要**: 研究采用KCNQ3抗体对癫痫患者脑组织样本进行Western blot及免疫组化分析，发现部分患者KCNQ3表达水平异常，提示其突变或表达失调可能与疾病病理相关。抗体特异性通过siRNA敲降实验确认。
4. **文献名称**: "Characterization of a novel monoclonal antibody against the KCNQ3 channel for functional studies"
**作者**: Li X, et al.
**摘要**: 本文报道了一种新型KCNQ3单克隆抗体的开发，通过ELISA和免疫沉淀验证其高亲和力与特异性。该抗体成功应用于流式细胞术检测转染细胞系中KCNQ3的表达，并用于探究通道功能与药物相互作用。
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**注**：上述文献为示例性质，实际引用时需根据具体研究内容检索真实文献(可通过PubMed或Google Scholar搜索关键词如“KCNQ3 antibody”或“KCNQ3 immunohistochemistry”)。若需真实文献，建议参考以下领域经典论文：
- Cooper EC & Jan LY (1998) 对KCNQ家族通道的早期定位研究。
- Pan Z et al. (2006) 对KCNQ2/KCNQ3复合体的功能分析。
The KCNQ3 antibody targets the KCNQ3 protein, a voltage-gated potassium channel subunit belonging to the Kv7 family (Kv7.3). KCNQ3 forms heterotetrameric channels, primarily with KCNQ2. to generate the neuronal M-current, a critical regulator of resting membrane potential and neuronal excitability. These channels are pivotal in controlling action potential firing and dampening excessive electrical activity in the brain. Mutations in the KCNQ3 gene are associated with neurological disorders such as benign familial neonatal epilepsy (BFNE) and developmental and epileptic encephalopathies (DEEs), highlighting its role in maintaining neuronal stability.
KCNQ3 antibodies are essential tools in neuroscience research, enabling the study of channel expression, localization, and function in both normal and pathological states. They are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to map KCNQ3 distribution in brain regions such as the hippocampus, cortex, and cerebellum. Additionally, these antibodies aid in investigating disease mechanisms, including channel dysfunction caused by genetic mutations or altered regulation (e.g., via PIP2 or neurotransmitter signaling).
Clinically, KCNQ3 antibodies may contribute to diagnostic assays or therapeutic development, particularly for epilepsy. However, challenges remain in ensuring antibody specificity due to homology among Kv7 subunits. Validated antibodies are crucial for distinguishing KCNQ3 from related isoforms (e.g., KCNQ2. KCNQ5) and advancing precision research. Ongoing studies continue to explore KCNQ3's interactions with regulatory proteins and its potential as a drug target for neuroexcitability disorders.
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