关键信息
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基因名
COL1A1
- 应用
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别名
COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
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种属
Human
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表达系统
HEK293
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P02452
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表达区间
Asp1219~Leu1464
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分子量
35kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Pro-COL1A1, or pro-alpha1(I) chain of type I collagen, is a crucial extracellular matrix protein that plays a significant role in maintaining the structural integrity of various tissues, particularly in bones and skin. Type I collagen is the most abundant collagen type in mammals and is predominantly found in connective tissues, providing tensile strength and supporting cellular functions. Impaired collagen synthesis or mutations in the COL1A1 gene can lead to severe connective tissue disorders, such as osteogenesis imperfecta and Ehlers-Danlos syndrome. Research into Pro-COL1A1 recombinant proteins is essential for understanding collagen biosynthesis and pathology. Recombinant production of this protein enables the study of its folding, post-translational modifications, and interactions with other extracellular matrix components. Moreover, Pro-COL1A1 serves as a valuable tool in regenerative medicine and tissue engineering, offering insights into potential therapeutic strategies for treating connective tissue disorders. By elucidating the mechanisms underlying collagen assembly and function, researchers aim to develop innovative treatments that might restore collagen-related deficiencies and improve patient outcomes in various fibrotic diseases and injuries.
