首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠碱性成纤维细胞生长因子(bFGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FGF2含量。FGF2即成纤维细胞生长因子2，它在细胞生长、增殖、分化等生理过程中发挥重要作用，与胚胎发育、组织修复等密切相关。研究机制上，它主要通过与细胞表面的受体结合，激活下游信号通路，调控基因表达，影响细胞行为。试剂盒检测范围为3.12 pg/mL-200 pg/mL，科研领域可用于探究生长因子在肿瘤微环境、干细胞分化、再生医学模型或炎症反应中的动态变化，为体外细胞实验、动物模型组织分析及生物标志物研究提供可靠工具，助力分子机制研究与药物开发。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fgf2 ELISA Kit; Fgf-2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit
缩写：FGF2
Uniprot No.：P15655
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of mouse bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 96 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.8952.8632.8792.7111002.4552.3642.4102.242501.9951.9761.9861.818251.5561.5321.5441.37612.51.0110.9680.9900.8226.250.4520.4270.4400.2723.120.2920.2840.2880.12000.1690.1670.168 | ||||||
货期：3-5 working days
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