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产品描述：人纤维连结蛋白III型域包含蛋白5(FNDC5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FNDC5含量。人纤维连结蛋白III型域包含蛋白5（FNDC5）是一种跨膜蛋白，其裂解产物鸢尾素（Irisin）能促进脂肪组织褐变，调节能量代谢。研究显示，FNDC5在骨骼肌中表达，通过影响脂肪细胞褐变和代谢，与肥胖和心血管疾病等代谢性疾病相关。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于体外培养细胞模型中蛋白表达水平检测、动物实验组织样本分析，以及临床前研究中血液样本的定量评估，为探究FNDC5在代谢调控、运动干预生理效应及能量稳态中的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fibronectin type III domain containing 5 ELISA Kit; Fibronectin type III domain-containing protein 5 ELISA Kit; Fibronectin type III repeat containing protein 2 ELISA Kit; Fibronectin type III repeat-containing protein 2 ELISA Kit; FNDC 5 ELISA Kit; Fndc5 ELISA Kit; FNDC5_HUMAN ELISA Kit; FRCP2 ELISA Kit; irisin ELISA Kit
缩写：FNDC5
Uniprot No.：Q8NAU1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FNDC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-91 | |||||
| 1:2 | Average % | 106 | ||||
| Range % | 101-109 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 85-92 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of human FNDC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 84-91 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 5002.1932.1862.1902.063 2501.4351.4591.4471.320 1250.8940.8790.8870.760 62.50.5410.5680.5550.428 31.20.3830.3620.3730.246 15.60.2870.2980.2930.166 7.80.2090.2210.2150.088 00.1260.1280.127 | ||||||
货期：3-5 working days
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