首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠卵泡抑素(FS)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FST含量。小鼠卵泡抑素（FS）是一种TGF-β超家族成员，主要在垂体前叶的滤泡星形细胞中产生，具有抑制促卵泡激素（FSH）的能力。它通过结合TGF-β家族配体阻止其与受体结合，从而调节多种生理过程，包括生殖、肌肉生长和脂肪棕色化等。试剂盒检测范围为0.05 ng/mL-10 ng/mL，支持多类型样本检测，可应用于小鼠模型中FS表达水平与卵巢功能、胚胎发育或骨骼生长调控的关联性研究，亦可用于探究病理条件下FS在代谢紊乱或组织修复中的作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FstFollistatin ELISA Kit; FS ELISA Kit; Activin-binding protein ELISA Kit
缩写：FST
Uniprot No.：P47931
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.05 ng/mL-10 ng/mL
灵敏度：0.025 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse follistatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 83-91 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 97-105 | |||||
回收率：
| The recovery of mouse follistatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 91 | 87-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 100.2210.2250.223 2.50.3420.3510.347 0.80.5330.5460.540 0.20.7980.7780.788 0.051.2441.2231.234 02.3172.1632.240 | ||||||
货期：3-5 working days
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