| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human MAP2K7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAP2K7抗体的3篇参考文献示例(注：以下为虚构示例，实际文献需通过学术数据库检索获取)：
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1. **文献名称**: *MAP2K7 Antibody Validation in Stress-Activated Protein Kinase Pathways*
**作者**: Smith A, et al.
**摘要**: 本研究验证了一种高特异性兔源MAP2K7多克隆抗体在Western blot和免疫组化中的应用，证实其在人肝癌细胞系中检测内源性MAP2K7蛋白的可靠性，并揭示了其在JNK通路激活中的作用。
2. **文献名称**: *Role of MAP2K7 in Neurodegenerative Disease: Antibody-Based Detection in Mouse Models*
**作者**: Chen L, et al.
**摘要**: 通过开发一种小鼠单克隆MAP2K7抗体，研究团队发现MAP2K7在阿尔茨海默病模型小鼠脑组织中表达异常，提示其可能通过调控tau蛋白磷酸化参与疾病进展。
3. **文献名称**: *MAP2K7 Inhibition via Antibody Blocking Suppresses Colorectal Cancer Metastasis*
**作者**: Wang Y, et al.
**摘要**: 使用人源化MAP2K7抗体阻断实验，证明抑制MAP2K7可显著降低结直肠癌细胞侵袭能力，表明该抗体在靶向治疗中的潜在应用价值。
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**提示**：实际研究中建议通过PubMed、Web of Science等平台检索关键词“MAP2K7 antibody”、“MEK7 antibody”或“MAP2K7 immunoassay”获取具体文献，并关注抗体货号(如CST #9154)及验证实验(如敲除验证、交叉反应性测试)。
The MAP2K7 antibody is a crucial tool for studying the mitogen-activated protein kinase kinase 7 (MAP2K7), also known as MKK7. a serine/threonine kinase within the JNK (c-Jun N-terminal kinase) signaling pathway. MAP2K7 functions as a dual-specificity kinase that activates JNK by phosphorylating its threonine and tyrosine residues. This pathway is pivotal in cellular responses to stress, cytokines, and growth factors, regulating processes like apoptosis, inflammation, and differentiation. Dysregulation of MAP2K7/JNK signaling is implicated in cancer, neurodegenerative diseases, and immune disorders.
The MAP2K7 antibody specifically detects endogenous MAP2K7 protein in various experimental applications, including Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP). Researchers use it to investigate MAP2K7 expression levels, post-translational modifications, and subcellular localization across tissues or cell lines. Due to alternative splicing, MAP2K7 exists in multiple isoforms, and antibody specificity for targeted variants must be validated.
In disease research, this antibody aids in identifying MAP2K7's role in tumor progression, where its activity may suppress or promote malignancy depending on context. It also helps explore MAP2K7's involvement in neuronal survival pathways and inflammatory responses. Commercial MAP2K7 antibodies are typically raised against conserved epitopes, often in human, mouse, or rat samples. Proper validation using knockout controls or siRNA is essential to confirm antibody reliability, as cross-reactivity with related kinases (e.g., MAP2K4) can occur. Overall, the MAP2K7 antibody is indispensable for dissecting JNK pathway dynamics in health and disease.
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